FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131424
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACGGACATCCGACATGCGAACAGAGGTTATAAGAATGATGGAA5000.3804480155831507No Hit
CGAGATGACGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGA3990.30359751643535426No Hit
CGAGATGACGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTA3770.28685780374969566No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC2960.22522522522522523No Hit
CGAGATGACCGAAATTTCTGGAGGGGTGAAAATGGACGAAGGACAAGGGT2720.20696372047723396No Hit
CGAGATGACGACACCCACGGTCGATTCGAGCCATGCCAGTTATCCCTGCA2310.17576698319941564No Hit
GATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT1880.14304845385926468No Hit
CGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGT1850.14076576576576577No Hit
CGAGATGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG1740.13239590942293644No Hit
CGAGATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA1710.13011322132943753No Hit
AGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA1620.12326515704894082No Hit
CGAGATGACGAGACATCCGACATGCGAACAGAGGTTATAAGAATGATGGA1590.12098246895544194No Hit
CGAGATGACGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGG1370.1042427562697833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGC750.095.08
CGCGCAC450.095.06
ACGATGT650.095.05
CGCTCAT353.274181E-1095.01
CGGATGA850.095.01
CTACGAC1650.095.06
GTGTCGT1900.095.02
CGTGTAT353.274181E-1095.01
GCGTGAC156.6047756E-495.02
GCTACGT156.6047756E-495.06
GCTACAC650.095.02
CGCGTCA500.095.06
AGCTACG156.6047756E-495.05
TATACGA301.2234523E-895.04
GTGACGT156.6047756E-495.04
ACTCACG750.095.07
TGAGCTA156.6047756E-495.03
CGATCGA1300.095.09
CTATCAC201.7380618E-594.999993
GCGCACT409.094947E-1294.999997
Produced by FastQC (version 0.11.5)