FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6760
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGTACGTACGTTTCGACC400.591715976331361No Hit
GTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCA260.38461538461538464No Hit
ATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTG210.3106508875739645No Hit
AGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCC150.22189349112426035No Hit
CCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCA140.20710059171597633No Hit
GTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTAT120.17751479289940827No Hit
CCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCC120.17751479289940827No Hit
GCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTC120.17751479289940827No Hit
CGGCGAAGCCGATCAAGGAATGGGGCATCACCTAGTTCTTGGTCTGCAAC110.16272189349112426No Hit
GGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGG110.16272189349112426No Hit
GATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGGC110.16272189349112426No Hit
GAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCG100.14792899408284024No Hit
CGCGCGATCCCCGTTCCCATTAAGGGCATTTTGGACAAAGCGTCTACGCT90.1331360946745562No Hit
AAGCCGATCAAGGAATGGGGCATCACCTAGTTCTTGGTCTGCAACTCGTT90.1331360946745562No Hit
CAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATC90.1331360946745562No Hit
ATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCAC90.1331360946745562No Hit
CATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCA90.1331360946745562No Hit
TCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGGCTGT80.1183431952662722No Hit
CGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTG80.1183431952662722No Hit
GTGCTGCCCCTTCCTCTTAGGGATTTCTGATCTCGGCGAAGCCGATCAAG80.1183431952662722No Hit
GGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCT70.10355029585798817No Hit
AGGGTGCTGCCCCTTCCTCTTAGGGATTTCTGATCTCGGCGAAGCCGATC70.10355029585798817No Hit
CTCCACTATCTGCTTTCCAGCACGTGTGGCTGTCTCGATGTCCAGACCGA70.10355029585798817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT254.1392923E-795.02
CGCGATA254.1392923E-795.03
GCGATAC301.2253804E-679.166674
CGATACA200.001985618471.255
CGCGCGA451.3601773E-552.7777751
GATACAC300.00988946747.56
Produced by FastQC (version 0.11.5)