FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33512
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGAT4691.399498687037479No Hit
ACGCGTGATCAGCAAAAGCAGGTCTATCAGAAACGAATGGGGGTGCAGAT4461.3308665552637862No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGA3901.1637622344234901No Hit
CGTACTCTCGCTATTGCCGCAAATATCATTGGGATCTTGCACTTGACATT3561.062306039627596No Hit
ACGCGTGATCAGTAGAAACAAGGCAGTTTTTTACTCCAGCTCTATGCTGA1390.41477679637144904No Hit
CGTACTCTCGCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTC1150.34316065886846503No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAA950.283480544282645No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT910.271544521365481No Hit
CGTACTCTCGCATAGACTACTACAGCTCTATGCTGACAAAATGACCATCG630.187992360945333No Hit
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT630.187992360945333No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTAGTTT610.182024349486751No Hit
CGTACTCTCGCATAGACTACTAAGGCCCTCCTTTCAGTCCGTATTTAAAG600.17904034375746No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTCTATCA530.158152303652423No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGTGTTT520.15516829792313203No Hit
CGTACTCTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTA410.122344234900931No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCCTATCA390.116376223442349No Hit
CGTACTCTCTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCA370.110408211983767No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA350.104440200525185No Hit
CGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAA350.104440200525185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACTA201.719121E-595.000017
ACTCACG201.719121E-595.000017
ACATACT201.719121E-595.000013
TAGAGTA254.522808E-795.000013
CGTGTCG500.095.000011
GTCTACT201.719121E-595.000016
CGAGATG201.719121E-595.000011
ACGATGT201.719121E-595.000015
CGATGTG201.719121E-595.000016
ATACTCA201.719121E-595.000015
GAGACGA201.719121E-595.000012
CGACATA254.522808E-795.000011
GTGTCGC301.1956217E-895.02
GTCGTGT700.095.07
TCGTGTG700.095.08
TATGAGT156.5614935E-495.03
ATACGAC353.1650416E-1095.09
CGTGTGC700.095.09
GCGCACT301.1956217E-895.07
CGTGTAT353.1650416E-1095.01
Produced by FastQC (version 0.11.5)