FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63205
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGA7561.1961079028557868No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAA4700.7436120560082272No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACC3730.5901431848746144No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT2610.41294201408116443No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGC2520.39870263428526226No Hit
CGAGTATATAGTACTGTGTCAACAGTGTCGGTTGAATTGTTCGCATGGTA1840.29111620916066766No Hit
CGAGTATATAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATTATT1670.264219602879519No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAGACTACTTGTCAAT1660.26263744956886326No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACAC1470.232576536666403No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTC1300.2056799303852543No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1290.2040977770745985No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTGAAAG1250.19776916383197532No Hit
GTGCTGCCCCTTCCTCTTAGGGATTTCTGATCTCGGCGAAGCCGATCAAG1210.1914405505893521No Hit
CGAGTATATAGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCA1120.17720117079344988No Hit
ACGCGTGATCAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCA960.15188671782295704No Hit
ACGCGTGATCAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAAT940.14872241120164545No Hit
CGAGTATATAGTACGCGTGATCAGCAAAAGCAGGGGAAAATAAAAACAAC930.14714025789098964No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTAGTTTT880.13922949133771062No Hit
CGAGTATATAGTACTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACT850.13448303140574322No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCTTTAATTGTCGTACTCCT770.1218258049204968No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT720.11391503836721778No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCCA710.11233288505656197No Hit
CGAGTATATAGTACTGTCTCTGCACACACACATCACTTTGCCGGTATCAG690.10916857843525038No Hit
ACGCGTGATCAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGAT660.10442211850328297No Hit
CGAGTATATAGTACTGTCACTACAGAGACATAAGCATTTTCATTCTGATA650.10283996519262716No Hit
CGAGTATATAGTACTGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT640.10125781188197137No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT640.10125781188197137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA850.095.000011
TAATACT156.5887737E-495.09
CTCACGC156.5887737E-495.08
GAGCTAC353.237801E-1095.04
CGAGTAG500.095.01
ATAGGAC156.5887737E-495.08
GTCTATC1250.095.02
CGTCTCG500.095.06
CGTCTAT1250.095.01
CGCGAGT450.095.09
TCGTGTG1350.095.08
TATCTAT156.5887737E-495.06
TACGATA156.5887737E-495.09
GTGTCGT500.095.02
CGAGGAT156.5887737E-495.01
TCTCGAC450.095.08
GTACTCA156.5887737E-495.09
GTATCTA156.5887737E-495.05
CATACGA1100.095.08
CGTGTGC1350.095.09
Produced by FastQC (version 0.11.5)