FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92071
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA3640.3953470691097088No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC3090.33561056141456047No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC2920.317146549945151No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT1970.21396530938080394No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1750.19007070630274464No Hit
CGTCAGTCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATT1690.1835539963723648No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1670.18138175972890486No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT1510.16400386658122534No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1310.14228150014662597No Hit
CGTCAGTCTCTGCGCGATCTCGGCTTTGAGGGGGCCTGACGGGATGATAG1220.13250643525105626No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGC1120.12164525203375656No Hit
ACGCGTGATCAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGA1110.12055913371202659No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT1020.11078406881645686No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGCCATTCTGTTTTCATGCCTGATT950.10318124056434709No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA940.10209512224261712No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT940.10209512224261712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG850.095.000011
GCGCGTC550.095.000015
GAGTACG201.735282E-595.000016
GCTATAC201.735282E-595.000012
CGCGCGT550.095.000014
CGACATA550.095.000011
CGAGAGT201.735282E-595.000011
TCGAGAC800.095.000013
CGACTAT201.735282E-595.000011
AGTAGAC850.095.000013
CGATAGT550.095.000011
GTACGCT1100.095.000015
GTCGATA500.095.02
CGACGTA156.5984327E-495.08
GAGTATA500.095.02
CTATAGT254.5867455E-795.09
CGTCTCG1150.095.06
ACGATGT353.255991E-1095.05
CGCTACA450.095.01
GTGTCGT1200.095.02
Produced by FastQC (version 0.11.5)