FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31900
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTAGTCGCGCTTCAATCGCTGGCCCAGCAGCCATGCCTTCTAAGCAGT1250.3918495297805642No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1200.3761755485893417No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA950.29780564263322884No Hit
ACGCGTGATCAGCAAAAGCAGGAATCACTCTAGAATTATGCCTTTGACAT610.19122257053291536No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT600.18808777429467086No Hit
CGCTAGTCGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACA580.18181818181818182No Hit
ACGCGTGATCAGCAAAAGCAGGCCGGACTCATAAACTGGGGCGATAACAG510.15987460815047022No Hit
CGCTAGTCGCGTTGGGGCAAGAAGGGGATTGAACTCAGCAATGAGATGGA430.13479623824451412No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT390.12225705329153606No Hit
ACGCGTGATCAGTAGAAACAAGGTCTGAGGAAGCAAGGCCCATAGTTGAC350.109717868338558No Hit
CGCTCGTTGCCTCCTTCATCTACCTGCTGGTCTCGGGGAACTCGCTGAAC340.10658307210031348No Hit
GGATTGAACTCAGCAATGAGATGGAAGTATGGAACTTAGAAGCTTACTAC330.10344827586206896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA201.717851E-595.000011
TACAGCT201.717851E-595.000014
GATACAG201.717851E-595.000012
AGCTACG201.717851E-595.000015
TCGTGTG201.717851E-595.000018
GCTACGT201.717851E-595.000016
GTGAGCT201.717851E-595.000012
GAGTAGA201.717851E-595.000012
CGAGTAG201.717851E-595.000011
TGAGCTA201.717851E-595.000013
ACAGCTA201.717851E-595.000015
GTGTCGT156.558585E-495.02
TCTAGAT156.558585E-495.05
TATGTAT156.558585E-495.03
ACGTAGA156.558585E-495.05
GTATATA254.5177876E-795.04
AGTATAC156.558585E-495.03
AGACGTA156.558585E-495.03
AGAGTAC254.5177876E-795.03
ACGAGAC254.5177876E-795.08
Produced by FastQC (version 0.11.5)