FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55812
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA3090.5536443775532144No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1780.31892782914068657No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1630.2920518884827636No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1260.22575790152655345No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA1150.20604887837740987No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT1130.20246541962302012No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1030.18454812585107144No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA880.15767218519314843No Hit
CACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGG730.1307962445352254No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT720.12900451515803052No Hit
CGTCGATATAGTCAGAGGTGACAAGATTGGTCTTGTCTTTAGCCATTCCA710.12721278578083564No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT700.1254210564036408No Hit
CGTCGATATAGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC700.1254210564036408No Hit
CGTCGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGA680.12183759764925106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG201.7292627E-595.000013
GTGTCGT950.095.000012
ACGCTGT409.094947E-1295.000017
GTCGTCT950.095.000014
CGCTACA201.7292627E-595.000011
GCTACGT201.7292627E-595.000016
ACTCACG201.7292627E-595.000017
ATACGTG201.7292627E-595.000019
AGACGTA201.7292627E-595.000013
GCTGTCG409.094947E-1295.000019
CGTACGC409.094947E-1295.000014
GCGCACT409.094947E-1295.000017
TCGTACG409.094947E-1295.000013
CTCACGC201.7292627E-595.000018
ACGCGTC201.7292627E-595.000017
CGCGTCG201.7292627E-595.000018
CGACTGC201.7292627E-595.000019
CGCGCAC409.094947E-1295.000016
GAGACGT201.7292627E-595.000012
GTACGCT409.094947E-1295.000015
Produced by FastQC (version 0.11.5)