FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80934
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC5680.701806410161366No Hit
CGACATACTTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAA3340.4126819383695357No Hit
CGACATACTCACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAA3300.40773963970642746No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2800.3459609064175748No Hit
CACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGG2260.27923987446561394No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA2230.2755331504682828No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT2160.26688412780784343No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT2140.26441297847628936No Hit
CGACATACTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATT2130.26317740381051224No Hit
CGACATACTCACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGG2050.25329280648429586No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1840.2273457385029777No Hit
CGACATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTC1570.1939852225269973No Hit
CGACATACTCACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTG1020.1260286159092594No Hit
CGACATACTCACGCGTGATCAGCAAAAGCAGGAGTTTAAAATGAATCCAA1010.12479304124348234No Hit
CGACATACTTACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTG1000.1235574665777053No Hit
ACGCGTGATCAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCG970.11985074258037413No Hit
CGACATACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTT950.11737959324882002No Hit
CGACATACTCACGGAAACCACTGACTGTCCAGCAGCAAAGTTGGTGTTGC940.11614401858304298No Hit
GTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAG890.10996614525415771No Hit
CCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAGGAATGG840.10378827192527244No Hit
CGACATACTTACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATT830.1025526972594954No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT810.10008154792794127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGG201.7340039E-595.000018
GAGTACA450.095.000014
CTGACGT450.095.000013
AGAGTAC450.095.000013
TATAGTC201.7340039E-595.000017
CGTCGAT201.7340039E-595.000011
CGAGAGT450.095.000011
CGACTAG409.094947E-1295.000019
AGTACAC450.095.000015
GATACAG254.5816705E-795.02
CTCACAC156.595516E-495.08
GTCGATA254.5816705E-795.02
AGATACG254.5816705E-795.07
GCGCGTC700.095.05
CGCTGAC500.095.01
GCTGACG500.095.02
TCGTGTG500.095.08
CGTAGAG353.255991E-1095.01
CATACTA353.255991E-1095.04
CGTGTGC500.095.09
Produced by FastQC (version 0.11.5)