FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87250
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAA4100.4699140401146132No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2430.27851002865329516No Hit
CGATAGTCGTACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGG2250.25787965616045844No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC2220.2544412607449857No Hit
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGC1880.2154727793696275No Hit
CGATAGTCGTACTCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCA1570.17994269340974212No Hit
CGATAGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCC1510.17306590257879656No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1320.1512893982808023No Hit
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTG1260.14441260744985673No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1130.12951289398280802No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1120.12836676217765042No Hit
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTT1090.12492836676217765No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA1020.11690544412607451No Hit
CGATAGTCGTACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGG930.10659025787965615No Hit
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA920.10544412607449856No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT920.10544412607449856No Hit
CGATAGTCGTACTCAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGAT890.1020057306590258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT700.095.01
CGAGTAG1050.095.01
GCGCGTC500.095.05
GTCTAGA254.5847264E-795.04
CGACAGC156.597269E-495.04
CTGACGT254.5847264E-795.03
ACGATGT301.218541E-895.05
AGACGAC301.218541E-895.06
TCGTGTG850.095.08
TCGGACT353.255991E-1095.07
CGCTCAT254.5847264E-795.01
CGTAGTA156.597269E-495.01
GTGTCGC600.095.02
CGTACTT254.5847264E-795.08
CGTACCC254.5847264E-795.08
CGTGTGC850.095.09
CGTGTAT600.095.01
CGAGATG500.095.01
CGATGTG301.218541E-895.06
CGCACTC650.095.08
Produced by FastQC (version 0.11.5)