FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15543
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2101.3510905230650454No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1631.0487035964742972No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1340.8621244290034099No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1080.6948465547191662No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT900.5790387955993052No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTTAAAG710.4567972720838963No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT650.41819468571060925No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT540.3474232773595831No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT480.30882069098629605No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT400.2573505758219134No Hit
CGCTATACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAA330.21231422505307856No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT300.19301293186643506No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA300.19301293186643506No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTGACAAA220.14154281670205238No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTAGTTTTTTACTC220.14154281670205238No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTAGTTTT210.13510905230650455No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT200.1286752879109567No Hit
CGCTATACGAAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTG180.11580775911986102No Hit
CGTATAGACGACTAGTACGTGATCAGCAAAAGCAGGTAGATATTTAAAGA180.11580775911986102No Hit
CGCTATACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAA180.11580775911986102No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGGTGACAAAAACAT170.10937399472431321No Hit
CGTGTATATACTCTAGCGAGTGATCAGTAGAAACAAGGTAGTTTTTTACT160.10294023032876537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA450.095.000015
CGTCTAT850.095.000011
ACAGCGA156.494369E-495.06
CGTCAGT156.494369E-495.01
GTGTCGT156.494369E-495.02
GTGTCGC156.494369E-495.02
CTATAGT156.494369E-495.09
CGATAGT156.494369E-495.01
GACAGCG156.494369E-495.05
AGTAGAC254.4077933E-795.03
GTCGTCT156.494369E-495.04
ATCTATA156.494369E-495.07
AGCTACG156.494369E-495.05
GTATCTA156.494369E-495.05
ACGTAGA156.494369E-495.05
GAGCTAC156.494369E-495.04
GTACACT156.494369E-495.08
TCGTGTG301.1532393E-895.08
CTGACGT156.494369E-495.03
TACACTC156.494369E-495.09
Produced by FastQC (version 0.11.5)