FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31355
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2700.861106681549992No Hit
CGTAGTATCTATAGTACTGCTAACGCGAAGTAGGTACAGATGCAATTGTC1990.6346675171424015No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTGTTT1880.5995853930792537No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1750.5581247010046244No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1630.5198532929357359No Hit
CGTAGTATCTATAGTACTGCTAAGCCCATACACACAAGCAGGCAGGCAGG1390.4433104767979589No Hit
CGTAGTATCTATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGAT1300.4146069207462925No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT1110.35401052463721894No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTATTT960.30617126455110827No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT930.29660341253388617No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT910.2902248445224047No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT880.2806569925051826No Hit
CGTAGTATCTATAGTACTGCTACCCCTCTTAGTTTGCATAGTTTCCCGTT740.23600701642481262No Hit
CGTAGTATCTATAGTACTGCTACCGCGAAGTAGGTACAGATGCAATTGTC730.23281773241907192No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC670.21368202838462766No Hit
CGTAGTATCTATAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATC660.21049274437888693No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT630.2009248923616648No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGGGTTT590.18816775633870195No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTCGTTT510.16265348429277626No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGC470.14989634826981343No Hit
CGTAGTATCTATAGTACTGCTAACGAGAAAGCTCTTATCTCTTGTTCTAC470.14989634826981343No Hit
CGTAGTATCTATAGTACTGCTAAAACGAGAAAGCTCTTATCTCTTGTTCT420.13394992824110988No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT410.13076064423536918No Hit
CGTAGTATCTATAGTACTGCTAAATCCATGTTGTTCGGGTCCCCATTCCC390.12438207622388774No Hit
CGTAGCAGTACTATAGATACTACGAGATCGGAAGAGCGTCGTGTAGGGAA380.12119279221814704Illumina Single End PCR Primer 1 (100% over 26bp)
CGTAGTATCTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATT380.12119279221814704No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTATTTT360.1148142242066656No Hit
CGTAGTATCTATAGTACTGCTACTCCCATTCGCTTCTGGTAGGCCTGCAA360.1148142242066656No Hit
CGTAGTATCTATAGTACTGCTAAGGCGAAGTAGGTACAGATGCAATTGTC360.1148142242066656No Hit
CGTAGTATCTATAGTACTGCTACGCCCATACACACAAGCAGGCAGGCAGG350.1116249402009249No Hit
CGTAGTATCTATAGTACTGCTATGGGGCTACCCCTCTTAGTTTGCATAGT350.1116249402009249No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAATGGACGAAGGACAAGG350.1116249402009249No Hit
CGTAGTATCTATAGTACTGCTAGAAACGAGAAAGCTCTTATCTCTTGTTC320.10205708818370277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATG254.5159504E-795.07
GTATGTA500.095.02
AATCTAT156.557517E-495.06
GTGTATA156.557517E-495.02
GTCGTGT450.095.07
CACGTCG450.095.04
ACGTAGA254.5159504E-795.05
CTGACGT156.557517E-495.03
TACACTC156.557517E-495.09
CGCTATC156.557517E-495.01
GCGTGAC156.557517E-495.02
GCTATCA156.557517E-495.02
ACTCACG254.5159504E-795.07
TCACGTC450.095.03
AGACGAC156.557517E-495.06
CGTACGC301.19307515E-895.04
CGTATGT500.095.01
CGTACAC156.557517E-495.07
GCTGACG156.557517E-495.02
CGAGTAG254.5159504E-795.01
Produced by FastQC (version 0.11.5)