FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77469
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACAGAGTAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT4260.5498973783061611No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA3920.5060088551549652No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1840.23751436058294284No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT1520.19620751526417018No Hit
CGACATACAGAGTAGTACGCGTGATCAGTAGAAACAAGGGGGTTTTTTAT1510.19491667634795853No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1280.16522738127509068No Hit
CGACATACAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAAT1220.1574823477778208No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1160.14973731428055093No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1110.1432831196994927No Hit
CGACATACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACA1060.13682892511843447No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT990.12779305270495295No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGC780.1006854354645084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA201.7335342E-595.000018
CTCACGC409.094947E-1295.000018
CGCGAGC201.7335342E-595.000011
GTGTCGT800.095.000012
GTCGTCT800.095.000014
TACTCAC409.094947E-1295.000016
ACTCACG409.094947E-1295.000017
GTACGAT201.7335342E-595.000018
GCGAGCG201.7335342E-595.000012
ATCTATA301.216722E-895.07
GTCGATA301.216722E-895.02
CTATCAC353.255991E-1095.03
CTATAGT301.216722E-895.09
CGTCTCG750.095.06
GCTGACG254.579815E-795.02
TCGTGTG650.095.08
TATCTAT301.216722E-895.06
ATATAGT353.255991E-1095.06
GTATCTA301.216722E-895.05
CGTGTGC650.095.09
Produced by FastQC (version 0.11.5)