FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78226
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGCGTGTAGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA67588.639071408483114Illumina Single End PCR Primer 1 (100% over 36bp)
CGCTACACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAA8411.0750901234883543No Hit
CGACGCGTGTAGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGA5940.7593383274103239Illumina Single End PCR Primer 1 (97% over 36bp)
CGCTACACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTC5310.6788024442001381No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA5260.6724107074374249No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC4640.5931531715797816No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC3320.4244113210441541No Hit
CGCTACACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTT2610.33364865901362717No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA1700.2173190499322476No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTTAAAG1510.19303045023393756No Hit
CGACGCGTGTAGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGA1470.18791706082376705Illumina Single End PCR Primer 1 (97% over 36bp)
CGCTACACGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT1430.18280367141359652No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1300.16618515583054227No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1300.16618515583054227No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT1240.15851507171528648No Hit
CACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGG1240.15851507171528648No Hit
CGCTACACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCAT1160.1482882928949454No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT1150.1470099455424028No Hit
CGACGCGTGTAGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTAGA1100.1406182087796896Illumina Single End PCR Primer 1 (97% over 36bp)
CGCTACACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTA1070.13678316672206173No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1010.12911308260680593No Hit
CGCTACACGCGTGATCAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCA990.12655638790172066No Hit
CGCTACACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGA940.12016465113900748No Hit
CGCTACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAA920.11760795643392223No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT910.1163296090813796No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT840.10738117761358117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCG1250.095.000016
GCGCACT409.094947E-1295.000017
ACTACGA800.095.000015
GTCGTCT1250.095.000014
TCGAGAC409.094947E-1295.000013
CGACTGC800.095.000019
GCTGTCG409.094947E-1295.000019
TACAGCT201.7336397E-595.000014
TGTCGCG409.094947E-1295.000013
TAGTCGC201.7336397E-595.000014
GTACGCT409.094947E-1295.000015
GAGCTAC301.2169039E-895.04
CGACGTA156.594684E-495.08
GAGTACG254.5802335E-795.06
GCTATCA156.594684E-495.02
GTCTCGA1350.095.07
CGACATA1700.095.01
CTGACGT156.594684E-495.03
CGCTGAC156.594684E-495.01
GAGACGT254.5802335E-795.02
Produced by FastQC (version 0.11.5)