FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14442
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1170.8101371001246366No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1030.7131976180584407No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA970.6716521257443567No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT820.5677883949591469No Hit
CGATACGATACACGCGTATGTACGGCGCCATCAAGGGAGCAATTTTACAA580.4016064257028112No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT450.31159119235562943No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA340.23542445644647556No Hit
CGATACGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTA340.23542445644647556No Hit
ACGCGTGATCAGTAGAAACAAGGTATTTTTTCATGAAGGACAAGTTAAAT310.21465171028943358No Hit
CGATACGATACACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTA270.18695471541337766No Hit
CGATACGATACACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCT270.18695471541337766No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT250.17310621797534967No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT220.15233347181830773No Hit
CGATACGATTCACACTCTGGATTTCCCAGGATCCAGCCAGCAATGTTACA210.14540922309929372No Hit
CGATACGATACACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATT210.14540922309929372No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC200.13848497438027974No Hit
ACGCGTGATCAGCAAAAGCAGGCCAAATATATTCAATATGGAGAGAATAA200.13848497438027974No Hit
CGATACGATACACGCGTATGTACGCCGCCATCAAGGGAGCAATTTTACAA170.11771222822323778No Hit
CGATACGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA150.1038637307852098No Hit
ACGCGTGATCAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAA150.1038637307852098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAG201.6857548E-595.000012
CGATAGT201.6857548E-595.000011
GTACTCA201.6857548E-595.000019
TATGAGT201.6857548E-595.000013
AGTACGA201.6857548E-595.000017
TCAGTCT201.6857548E-595.000013
GAGTACG201.6857548E-595.000016
CGTACTC201.6857548E-595.000018
CTACGAC201.6857548E-595.000016
TCGTACT201.6857548E-595.000017
TGAGTAC201.6857548E-595.000015
GATAGTC201.6857548E-595.000012
ACTACGA201.6857548E-595.000015
GTACGAT201.6857548E-595.000018
GTGTCGT156.484859E-495.02
GTCGTCT156.484859E-495.04
TCGCGCA254.3916225E-795.05
ATACGTA156.484859E-495.06
TCTCGAC156.484859E-495.08
TCGTCTC156.484859E-495.05
Produced by FastQC (version 0.11.5)