FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21753
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT2351.0803107617340137No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT1500.6895600606812854No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA1250.5746333839010711No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC710.3263917620558084No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT670.30800349377097413No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA530.24364455477405414No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC520.2390474877028456No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT500.22985335356042846No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT450.2068680182043856No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT410.18847974991955133No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC350.1608973474922999No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA290.1333149450650485No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT270.12412081092263137No Hit
ACGCGTGATCAGCAAAAGCAGGCGTTTAAAATGAATCCAAACCAAAAGAT250.11492667678021423No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA230.10573254263779709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT254.4690205E-795.000011
ACTCACG254.4690205E-795.000017
ATAGTCG254.4690205E-795.000013
CTCACGC254.4690205E-795.000018
CGTCACG156.530214E-495.01
GTGTCGC156.530214E-495.02
GTCACGT156.530214E-495.02
ACGTCGT156.530214E-495.05
GTCGCGC156.530214E-495.04
CACGTCG156.530214E-495.04
TCGCGCA156.530214E-495.05
CGTAGTA156.530214E-495.01
GTACAGC156.530214E-495.09
TCGTGTG156.530214E-495.08
TCTACTA156.530214E-495.07
GCGTGAC156.530214E-495.02
TCACGTC156.530214E-495.03
GTGACGT156.530214E-495.04
GTCTATA156.530214E-495.09
CGTGTGC156.530214E-495.09
Produced by FastQC (version 0.11.5)