FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATCCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGATA50.463821892393321No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA50.463821892393321No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC40.3710575139146568No Hit
TCATTGCAGCAAATATCATTGGGATCTTGCACCTGATATTGTGGATTACT30.27829313543599254No Hit
CGGCTTTGAGGGGGCCTGACACGACGTATGTCGCGCGCGAGATCGGAAGA30.27829313543599254No Hit
AGTGATCCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGAT30.27829313543599254No Hit
CGGCTTTGAGGGGGCCGGACACGACGTATGTCGCGCGCGAGATCGGAAGA30.27829313543599254No Hit
ACGCGTGATCAGTAGAAACAAGGTGTTTTTTATCATTAAATAAGCTGAAA30.27829313543599254No Hit
ATGCAGCGATTCAAGTGATCCTCTCGTCATTGCAGCAAATATCATTGGGA30.27829313543599254No Hit
TCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAG20.1855287569573284No Hit
GGGTTTTCGGCTGAATCAAGAAAATTGCTTCTCATTGTTCAGGCACTTAG20.1855287569573284No Hit
TGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGTTTCCCAA20.1855287569573284No Hit
AAGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCGTCATTG20.1855287569573284No Hit
CGTCTATCATACGACTGTCTACACACGCGTGATCAGTAGAAACAAGGTAG20.1855287569573284No Hit
GTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACGGTTTGAAAAGA20.1855287569573284No Hit
AATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATG20.1855287569573284No Hit
CAGCAAATATCATTGGGATCTTGCACCTGATATTGTGGATTACTGATCGT20.1855287569573284No Hit
AAGATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTT20.1855287569573284No Hit
CGTCGCGCGTCACGTATGACTGAAACAAGGTACTTTTTTGGACAGTATGG20.1855287569573284No Hit
AGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCC20.1855287569573284No Hit
CAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTCAGGATATAGCGGG20.1855287569573284No Hit
AGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGT20.1855287569573284No Hit
TAAGTGTCTCGCTGGATCCCTCTTTCAAGATCCATTCCACGATTTGTTTC20.1855287569573284No Hit
CAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATC20.1855287569573284No Hit
TTTACCCAAGTGTTGTTTTCATAAGTAATGACGCTTTGATTGCATGTTTC20.1855287569573284No Hit
TGGATTAGTCGTCCATCATAATCACTGAGTTTGAGTTCAGTGCACATTTG20.1855287569573284No Hit
TGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACGGTTTGA20.1855287569573284No Hit
GATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCAT20.1855287569573284No Hit
TGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGGTGTTGCC20.1855287569573284No Hit
CCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCAGTCTCTGCGCG20.1855287569573284No Hit
TATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAG20.1855287569573284No Hit
GTGAATCAGCAATCTGTTCACAAGTGGCACACACTAGACCAAAAGCAGCT20.1855287569573284No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC20.1855287569573284No Hit
AGAATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGC20.1855287569573284No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG20.1855287569573284No Hit
AATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCGTCATTGCAGCA20.1855287569573284No Hit
ATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATGA20.1855287569573284No Hit
CGGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAG20.1855287569573284No Hit
CAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCT20.1855287569573284No Hit
CCTAATTATTACTATGAGGAATGCTCCAGTTATCCCTGCACACACATGTG20.1855287569573284No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT20.1855287569573284No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)