FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52586
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA3730.7093142661544898No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC3570.6788879169360666No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2930.557182520062374No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1870.3556079564903206No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1660.31567337314114025No Hit
ACGCGTGATCAGTAGAAACAAGGCAGTTTTTTACTCTAGCTCTATGTTGA1500.28524702392271706No Hit
CGAGATGAGTCTATATACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT1250.2377058532689309No Hit
ACGCGTGATCAGCAAAAGCAGGTGACAAAAACATAATGGACTCCAACACC1220.23200091279047655No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT1200.22819761913817366No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT990.18826303578899328No Hit
CGAGATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGT980.18636138896284182No Hit
CGAGATGAGTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCAT910.1730498611797817No Hit
ACGCGTGATCAGCAAAAGCAGGCAGATATTTAAAGATGAGTCTTCTAACC840.15973833339672155No Hit
CGAGATGAGTCTATATACGCGTGATCAGTAGAAACAAGGGGGTTTTTTAT820.15593503974441866No Hit
ACGCGTGATCAGCAAAAGCAGGCGACAAAAACATAATGGACTCCAACACC780.14832845243981288No Hit
ACGCGTGATCAGCAAAAGCAGGTTGACAAAAACATAATGGACTCCAACAC720.13691857148290418No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT650.12360704369984406No Hit
ACGCGTGATCAGCAAAAGCAGGCGCATTGACTGAAGATCCAGATGAAGGC640.12170539687369263No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA630.11980375004754117No Hit
CGAGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGA610.11600045639523827No Hit
GATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA570.10839386909063248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGT1000.095.000017
GTCGTCT254.5591696E-795.000014
GCGCGTC409.094947E-1295.000015
GCGTGTA201.728319E-595.000019
CAGAGTA254.5591696E-795.000018
ACTCACG500.095.000017
TCTCGAC201.728319E-595.000018
AGACGAC409.094947E-1295.000016
GTCTCGA201.728319E-595.000017
CGTACGC900.095.000014
GCGTCAC409.094947E-1295.000017
CGTGTCA201.728319E-595.000014
CGACGAG254.5591696E-795.000011
CTCACGC500.095.000018
CGCGCGT450.095.000014
TAGACGT500.095.000019
CGCGTCA409.094947E-1295.000016
CGACTAG409.094947E-1295.000019
CGCGAGT201.728319E-595.000019
GTATGTA700.095.02
Produced by FastQC (version 0.11.5)