FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59521
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAGAACCAAAACC1840.3091345911527024No Hit
CCAAAAGAACCAAAACCAAAGGCCTTAATCCCATTATATTTCTTGAAGAA950.15960753347558004No Hit
ACGCGTGATCAGCAAAAGCAGGGCCAAACTGCCTCATCACGGGACTGTCT850.14280674047815056No Hit
ACGCGTGATCAGCAAAAGCAGGCATGTATGTCCAGCATAAACCTGCAGAG750.12600594748072108No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTTAATGATATGGGTCC650.10920515448329161No Hit
CGCGTCTAGATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATG640.10752507518354867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC156.586858E-495.04
GATAATC301.2118107E-895.09
TAGACGA301.2118107E-895.05
TAGGTAC156.586858E-495.07
CGCATCT301.2118107E-895.01
CGCCTCT156.586858E-495.01
CTAGTCG156.586858E-495.03
CGCTAGT156.586858E-495.01
CGCTACA156.586858E-495.01
GTGTCGC156.586858E-495.02
CATACGA353.237801E-1095.08
ACGTCGT254.5666638E-795.09
ACGTCAC156.586858E-495.08
TAGATAG156.586858E-495.07
CTACACG156.586858E-495.03
GCTACGT301.2118107E-895.06
GCTACAC156.586858E-495.02
ATACGTA301.2118107E-895.06
CACGTCT254.5666638E-795.01
CGACTAT156.586858E-495.01
Produced by FastQC (version 0.11.5)