FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17270
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA530.30689056166763173No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA250.1447596988998263No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT220.12738853503184713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCA254.4293301E-795.000019
GTGAGCT254.4293301E-795.000012
CGTGAGC254.4293301E-795.000011
TGAGCTA254.4293301E-795.000013
GTGTCGC156.507011E-495.02
ATAGTCG301.1610609E-895.03
AGTCGTA301.1610609E-895.05
GCACTCG156.507011E-495.09
TGTCGCG156.507011E-495.03
TCGCGCG201.6953774E-594.999993
GCGCGTC201.6953774E-594.999995
GCGTCAC201.6953774E-594.999997
CGTGTCG201.6953774E-594.999991
CGCGCGT201.6953774E-594.999994
CGCGTCA201.6953774E-594.999996
CGTCGTA2250.088.6666641
CGATAGT353.3851393E-881.4285661
TAGTCGT353.3851393E-881.4285664
AGCTACG301.3109293E-679.166675
GCTACGT301.3109293E-679.166676
Produced by FastQC (version 0.11.5)