FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT1330.9728622631848439No Hit
ACGCGTGATCAGTAGAAACAAGGTATTTTTTCATGAAGGACAAGTTAAAT1200.8777704630239194No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC730.5339770316728841No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT300.21944261575597984No Hit
CACCATGGACACAGTGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATG220.16092458488771855No Hit
ACGCGTGATCAGCAAAAGCAGGTAAACCATTTGAATGGATGTCAATCCGA220.16092458488771855No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT200.1462950771706532No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT170.12435081559505523No Hit
CGACGACAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT160.11703606173652256No Hit
ACGCGTGATCAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGA140.10240655401945725No Hit
ACGCGTGATCAGCAAAAGCAGGCATTTTTTCATGAAGGACAAGTTAAATT140.10240655401945725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT450.095.06
GTCGCAC156.4775016E-495.04
TCGCGTG450.095.07
TAGTCGC450.095.04
GCGTGTA450.095.09
CGCTAGT450.095.01
GCACGCG156.4775016E-495.07
GGCTTTG254.379126E-795.02
AGTCGCA156.4775016E-495.03
CTAGTCG450.095.03
CGGCTTT254.379126E-795.01
AGACTAC156.4775016E-495.03
GCTTTGA156.4775016E-495.03
CGCGTGT450.095.08
TAGACGA156.4775016E-495.05
GAGACTA156.4775016E-495.02
CGTCTAT301.1426891E-895.01
GTATGAG201.682563E-594.999992
TATGTAT201.682563E-594.999993
ACGTAGA201.682563E-594.999995
Produced by FastQC (version 0.11.5)