FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301754
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT16060.5322216109811304No Hit
CGTCTATCATCCGACATGCGAACAGAGGTTATAAGAATGATGGAAAGTGC12620.4182214651669903No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT11450.3794481597592741No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTT9370.31051783903444524No Hit
CGTCTATCATACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCC8540.28301198989905685No Hit
CGTCTATCATACAACTACTGCTTGAAGTAGAACAAGAGATAAGAGCTTTC6680.22137237617396952No Hit
CGTCTATCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCC5820.19287233972043452No Hit
CGTCTATCATACGACTGTCTACATTCCCATTTAGGGCATTTTGGATAAAG5480.18160488344810674No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA4990.1653664905850461No Hit
GGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACG4680.1550932216308649No Hit
CGTCTATCATACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGA4510.149459493494701No Hit
GGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG4510.149459493494701No Hit
CGTCTATCATACGACATCCGACATGCGAACAGAGGTTATAAGAATGATGG3780.12526760208646778No Hit
CGTCTATCATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGAC3410.11300595849599343No Hit
CAGATGCAGCGATTCAAGTGATCCTCTCGTCATTGCAGCAAATATCATTG3310.1096920007688382No Hit
GTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTTT3260.10803502190526058No Hit
TGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGTCTT3130.10372687685995877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG2500.095.000011
CGTCAGT1800.095.000011
GTCGATC156.6131563E-495.02
GAGTACA1150.095.04
GTCTAGA700.095.04
TCGATCA156.6131563E-495.03
TGTGATA301.2289092E-895.05
ACGATGT550.095.05
CATAGTC353.2923708E-1095.03
GTGTCGT2100.095.02
CGTCGTA1100.095.01
CGTCGAT750.095.01
GCTACAC1450.095.02
CGCGTCA550.095.06
ACGAGTC600.095.03
ACATACG156.6131563E-495.07
CGTTTAT301.2289092E-895.01
TGTCGCG353.2923708E-1095.03
CGATAGT2400.095.01
AGTACGA700.095.07
Produced by FastQC (version 0.11.5)