FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68368
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGGTGTTTTCTGTTAGATGCCAGGAGGT6380.9331851158436695No Hit
ACGCGTGATCAGTAGAAACAAGGGGCATCACTGTCACAGTGGTGAGTGAT4140.6055464544816288No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTGAACTTTGTTTTCTTGATTTGAA3010.4402644512052422No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAGCAAGAGAG1510.22086356190030423No Hit
ACGCGTGATCAGTAGAAACAAGGGTGAGAAGATGTAGGAGCTGTCCACCC1210.17698338403931665No Hit
ACGCGTGATCAGCAAAAGCAGGGACTCTGTCTTAGTCACTACTTCATCCC1140.16674467587175287No Hit
ACGCGTGATCAGCAAAAGCAGGAGCAAGAGAGACTGAGTGTGAGACACCA1070.1565059677041891No Hit
ACGCGTGATCAGTAGAAACAAGGGCCCTTCATGTTTCTCACACTCCCTTG1050.15358062251345658No Hit
ACGCGTGATCAGTAGAAACAAGGTGATCTTCCAGGAGCTAATGGTAGTGC1040.15211794991809033No Hit
CGTAGAGTATCTGAGTAACGTGTGAAAAACAGATTTTAAGTTGTAAGTGC970.14187924175052657No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGTTGAACT890.13017786098759654No Hit
ACGCGTGATCAGCAAAAGCAGGAATCACTCTAGAATTATGCCTTTGACAT820.11993915282003276No Hit
CGTAGAGTATCTGAGTATACGCCGGCGCCAAACCTTATGTGAAATTGTAA780.11408846243856775No Hit
ACGCGTGATCAGTAGAAACAAGGAGATCTTCAGCAAATCCTCACTTTTTA770.11262578984320151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACA254.5739944E-795.000011
GCTACTA254.5739944E-795.000018
CGAGTCG156.591095E-495.04
CGACGAG156.591095E-495.01
TAGACGA353.237801E-1095.05
GTCTAGA156.591095E-495.04
ACGATGT156.591095E-495.05
ACGATCG156.591095E-495.08
CATAGTC156.591095E-495.03
GTAGACG156.591095E-495.04
TCTCGAC156.591095E-495.08
CATACGA353.237801E-1095.08
GAGTTTC156.591095E-495.05
CGTCGTA156.591095E-495.01
CTCGACG156.591095E-495.09
CGCGTCT156.591095E-495.01
CGATCGA156.591095E-495.09
AAGTATC201.7320677E-594.999995
GAAGAGT201.7320677E-594.999992
CGACGTA201.7320677E-594.999998
Produced by FastQC (version 0.11.5)