FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20403
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGTTGATGTGCTAAATGTCAATTATTTCAA470.2303582806450032No Hit
ACGCGTGATCAGCAAAAGCAGGCTGATGTGCTAAATGTCAATTATTTCAA350.17154340048032152No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT340.16664216046659805No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA330.16174092045287458No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA320.15683968043915109No Hit
CGCATAGTCGTACGTATACATCCGGCCATCTGTTCCATAGCCTTTGCCGT250.12253100034308681No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC210.10292604028819291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGT156.524324E-495.01
ACGTCAC156.524324E-495.08
GTCGCGA156.524324E-495.07
CGTAGAG156.524324E-495.01
GAGTCGC156.524324E-495.05
ATAATCG353.092282E-1095.04
GTGAGCT301.1719749E-895.02
GACATAC156.524324E-495.02
AATAGTC156.524324E-495.03
ACGAGTC156.524324E-495.03
TACGTCA156.524324E-495.07
ACATAGT353.092282E-1095.02
AGTCGCG156.524324E-495.06
CGTACGC156.524324E-495.09
CGAGTCG156.524324E-495.04
TAGAGTA156.524324E-495.03
CGAGATG156.524324E-495.01
CGTGAGC301.1719749E-895.01
CGCACGT156.524324E-495.09
CGCATAA353.092282E-1095.01
Produced by FastQC (version 0.11.5)