FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60792
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA4360.7171996315304645No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC2470.40630346098170816No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT2400.3947887879984209No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA1900.31254112383208316No Hit
ACGCGTGATCAGTAGAAACAAGGTGATCTTCCAGGAGCTAATGGTAGTGC1800.2960915909988156No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT1430.23522831951572576No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT1390.22864850638241874No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1270.20890906698249767No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT970.1595604684826951No Hit
CGATACAGCTACTACTACTCTAATTCGCTTCTGGTAGGCCTGCAAATTTT780.1283063560994868No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT770.12666140281616No Hit
ACGCGTGATCAGTAGAAACAAGGCACTTTTTTGGACAGTATGGATAGCAA730.12008158968285301No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTCGTTT690.11350177654954599No Hit
CGATACAGCTACTACTACTCTAACGCGAAGTAGGTACAGATGCAATTGTC640.10527701013291224No Hit
CGATACAGCTACTACTACTCTACGTCCCCTTGCCCAATTAGCACATTAGC610.10034215028293196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGC254.567828E-795.000018
GTCTCGA500.095.000017
CTGACGT254.567828E-795.000013
CGCTGAC254.567828E-795.000011
ATATAGT254.567828E-795.000016
CGCTACA254.567828E-795.000011
CGTACAC254.567828E-795.000017
CGAGAGT254.567828E-795.000011
ACTCACG254.567828E-795.000017
GATACAT156.587531E-495.02
GTCGATA156.587531E-495.02
CGAGTCG600.095.04
CGACGAG600.095.01
GAGTCGC600.095.05
GAGTACG156.587531E-495.06
CGTCTAT1100.095.01
TGAGTAC156.587531E-495.05
AGACGTA156.587531E-495.03
GAGACGT156.587531E-495.02
ACTACGA156.587531E-495.05
Produced by FastQC (version 0.11.5)