FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12212
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1321.0809040288241074No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA990.8106780216180806No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT750.6141500163773338No Hit
ACGCGTGATCAGCAAAAGCAGGTTGATGTGCTAAATGTCAATTATTTCAA630.5158860137569604No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA520.4258106780216181No Hit
ACGCGTGATCAGCAAAAGCAGGCTGATGTGCTAAATGTCAATTATTTCAA460.37667867671143135No Hit
ACGCGTGATCAGTAGAAACAAGGTGATCTTCCAGGAGCTAATGGTAGTGC320.26203734032099574No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT310.2538486734359646No Hit
ACGCGTGATCAGTAGAAACAAGGCAGTTTTTTACTCTAGCTCTATGTTGA300.2456600065509335No Hit
CGCGTATACTCACACAAAATCCTCAGAAGGTAGAGGATGAGGAAACACTT250.20471667212577793No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC240.1965280052407468No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC220.1801506714706846No Hit
CGCGTATACTCTTTCTCACACACATGTCATGCTTTGTTTTCCTTATAGCA200.16377333770062236No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTCGTTTT170.13920733704552898No Hit
CGCGTATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCA140.11464133639043562No Hit
CGCGAGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCC140.11464133639043562No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA130.10645266950540452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAC301.1323209E-895.06
GTAGAGT156.4607314E-495.02
CGATACA301.1323209E-895.01
TACAGCT301.1323209E-895.04
AGCTACT301.1323209E-895.07
TACGTAC301.1323209E-895.07
TCTCGAC156.4607314E-495.08
TATCATA254.3507134E-795.05
GTCTATC254.3507134E-795.02
TAGAGTA156.4607314E-495.03
ATCATAC254.3507134E-795.06
GTCTACT156.4607314E-495.06
CGTCGCG156.4607314E-495.01
GCGTATA1300.095.02
CGAGATG156.4607314E-495.01
CATACGA254.3507134E-795.08
ATACAGC301.1323209E-895.03
CGCGTAT1350.095.01
CGTCTAC156.4607314E-495.05
GAGATGA156.4607314E-495.02
Produced by FastQC (version 0.11.5)