FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249581
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGGCATCACTGTCACAGTGGTGAGTGAT23320.9343659974116619No Hit
ACGCGTGATCAGTAGAAACAAGGGGTGTTTTCTGTTAGATGCCAGGAGGT9830.39386010954359507No Hit
ACGCGTGATCAGTAGAAACAAGGGCCCTTCATGTTTCTCACACTCCCTTG6550.2624398491872378No Hit
ACGCGTGATCAGTAGAAACAAGGACTGTAGCAGCTACATTTTATGCTAAT5420.2171639668083708No Hit
CGAGCTCTATACGTGTAGTCTCTATGTTGACAAAATGACCATCGTCAACA4830.19352434680524558No Hit
ACGCGTGATCAGCAAAAGCAGGCCCTGTACATGGAATGCTATCCTCTGTG4000.1602686101906796No Hit
ACGCGTGATCAGCAAAAGCAGGAATCACTCTAGAATTATGCCTTTGACAT3930.15746390951234268No Hit
ACGCGTGATCAGTAGAAACAAGGGTGAGAAGATGTAGGAGCTGTCCACCC3600.14424174917161162No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTGAACTTTGTTTTCTTGATTTGAA2940.1177974284901495No Hit
CGAGCTCTATACGTGTAGTCTCCTTCCTCCCTCATCCCCACTTCACTGAA2660.10657862577680191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTA156.611803E-495.06
ATGTACG409.094947E-1294.999998
TACGCAC201.7411443E-594.999997
CGAGTAT51400.093.244161
CGTCGTA1900.092.51
TAGACGA850.089.4117665
CGCGCAC850.089.4117666
GTGTCGC850.089.4117662
CATACGA1650.089.242428
CGAGCTC58300.088.807891
ATACGAC1800.087.0833369
CGTCAGT550.086.363641
GAGTATA56850.086.310462
GAGCTCT60400.086.113422
ACTACGA500.085.55
CGATCGA500.085.59
CGAGGAT1500.085.499991
CGTGTCG1950.085.256411
CGACGAG452.5465852E-1184.444451
GGATACG1350.084.444444
Produced by FastQC (version 0.11.5)