FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455203
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGTATAGACGTGATACTGAAACGAGAAAGCTCTTATCTCTTGTTCT36770.8077714777802432No Hit
CGTATGTATAGACGTGATACTGAAGAGCTTCCCAGCACTGGGATGCTCTT31660.6955138696361842No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGACACAGTAAACAGAACACA30320.6660764538019301No Hit
CGTATGTATAGACGTGATACTGGCGGGGAGCGCCAGGATGCCACAGAAAT29740.6533348857542679No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGAACAGGAACAGGATACACC21560.47363483984068644No Hit
CGTATGTATAGACGTGATACTGGACCACAACTGCCTGTCTTATCATTAGG18990.41717651245707954No Hit
CGTATGTATAGACGTGATACTGATGCAATTGTCGTTCTAAGTGTCTCGCT15410.3385302820939229No Hit
CGTATGTATAGACGTGATACTGTTTTCTTAGGGTCCTTCCCAGCACTGGG13460.2956922515888516No Hit
CGTATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTG11530.2532935854992168No Hit
CGTATGTATAGACGTGATACTGAAAAGGGAAAGATAGTCAAATCAGTCGA11260.24736216589082233No Hit
CGTATGTATAGACGTGATACTGAAGATCTTGTATGAGGCCTGTCCATCAC10120.22231839421093447No Hit
CGTATGTATAGACGTGATACTGCAAGCCCATACACACAAGCAGGCAGGCA9080.19947144460822971No Hit
CGTATGTATAGACGTGATACTGAAACAAGGCATTTTTTCATGAAGGACAA8440.1854117833142576No Hit
CGTATGTATAGACGTGATACTGAACAAAACTCCCGCTATATCCTGACCAC7660.16827657111222905No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGCATTTTTTCATGAAGGAC7220.1586105539726232No Hit
CGTATGTATAGACGTGATACTGAGGCCTATTCAACTGGTGCACTTGCCAG7110.15619404968772174No Hit
CGTATGTATAGACGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTAC6890.15136104111791882No Hit
CGTATGTATAGACGTGATACTGTTTACACCACAAAAGGATATGCTGCTCC6820.14982326566389062No Hit
CGTATGTATAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAG6810.1496035834561723No Hit
CGTATGTATAGACGTGATACTGATCCCCTCAGAATGAGTGCTGACCGTGC6700.14718707917127083No Hit
CGTATGTATAGACGTGATACTGTTACATTCTTTTCTAGTACTGTGTCTAC6520.14323279943234118No Hit
CGTATGTATAGACGTGATACTGTTTAAGGTCTCCAACATCTTTGCAGTCA6450.14169502397831296No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAAAGCACCATCC6350.13949820190112983No Hit
CGTATGTATAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGC6080.13356678229273533No Hit
CGTATGTATAGACGTGATACTGATTAGCAACCTCCATGGCCTCTGCTGCC6000.1318093246309888No Hit
CGTATGTATAGACGTGATACTGGACGGATCATGATCTCAGAGAACTCTTC5890.12939282034608734No Hit
CGTATGTATAGACGTGATACTGAAGCGAATGGGAGTGCAGATGCAGCGAT5850.1285140915152141No Hit
CGTATGTATAGACGTGATACTGGTGGGGAGCGCCAGGATGCCACAGAAAT5470.12016616762191813No Hit
CGTATGTATAGACGTGATACTGAGCTCAATTGCTCTCTTAGCTCCTCATA5390.11840870996017161No Hit
CGTATGTATAGACGTGATACTGAAATGGGAGGCTGGTGTTTATAGCACCC5380.1181890277524533No Hit
CGTATGTATAGACGTGATACTGCTGAGCTTTGGGTATGAATTTCCTTTTT5350.11752998112929836No Hit
CGTATGTATAGACGTGATACTGGGAGGCTGGTGTTTATAGCACCCTTGGG4900.1076442817819742No Hit
CGTATGTATAGACGTGATTTTGATTCCCAAGTTGAATTGAGTGGCTAATC4880.10720491736653757No Hit
CGTATGTATAGACGTGATACTGATGGACGACTAAAGCAGAAAGCACCATC4760.10456873087391778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG500.095.06
ACGATGT450.095.05
TCGTGTG254.6161585E-795.08
CACTACT301.2303644E-895.07
CGTAAGT301.2303644E-895.01
TCGCGCG450.095.03
CGTGAGC353.2923708E-1094.999991
TAGGCGT353.2923708E-1094.999999
GCTACGT409.094947E-1294.999996
GCTACAC700.094.999992
CACGTCG353.2923708E-1094.999994
CGTATGT280050.094.287631
GTATGTA283700.092.8736342
CGTATGG1350.091.481491
GTATAGA283400.090.910376
ATGTATA288800.090.707234
GTGTCGT2200.090.6818162
CGTCGTA1100.090.6818161
TATAGAC282500.090.678767
TAGACGT277250.090.596949
Produced by FastQC (version 0.11.5)