FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30831
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGACTGTCCAGCAGCAAAGTTGGTGTTGCTGATGTTAACATATGTC540.17514838960786222No Hit
CAGCAAAGTTGGTGTTGCTGATGTTAACATATGTCTGATTTACCCAAGTG330.10703512698258247No Hit
GTGTTGTTTTCATAAGTAATGACGCTTTGATTGCATGTTTCAATCTGATT330.10703512698258247No Hit
GTTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTG330.10703512698258247No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC320.10379163828614059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT254.5140405E-795.000011
GTCGTCT500.095.000014
CAGAGTA254.5140405E-795.000018
GCGTGAC500.095.000012
ACTCACG254.5140405E-795.000017
ATAGTCG254.5140405E-795.000013
TCTCGAC500.095.000018
AGTCGTA254.5140405E-795.000015
GAGTACA254.5140405E-795.000014
GTCTCGA500.095.000017
CGAGTAG254.5140405E-795.000011
CGAGATG254.5140405E-795.000011
CGAGAGT254.5140405E-795.000011
CATACTC254.5140405E-795.000014
TATAGTC254.5140405E-795.000017
CGTCTCG500.095.000016
ATATAGT254.5140405E-795.000016
CGCTGAC2050.095.01
CCGTGAC156.556411E-495.01
CTATAGT156.556411E-495.09
Produced by FastQC (version 0.11.5)