FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59447
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATACTC1270.21363567547563375No Hit
CGCTGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAAT1260.21195350480259725No Hit
CGCTGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC1220.20522482211045132No Hit
CGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT900.1513953605732838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT201.7301882E-595.000013
AGATCGT201.7301882E-595.000014
ACGTTCA201.7301882E-595.000016
GCGCGTC201.7301882E-595.000015
GACGTTC201.7301882E-595.000015
CGCGCAC409.094947E-1295.000016
TGACGTT201.7301882E-595.000014
CGTTCAC201.7301882E-595.000017
CGTAGTA201.7301882E-595.000011
CGTAGAT201.7301882E-595.000011
CGCTACA201.7301882E-595.000011
GTGTCGC409.094947E-1295.000012
CCGACGT201.7301882E-595.000013
ACGTACC201.7301882E-595.000016
CGCGTCG201.7301882E-595.000018
CGCGTCA201.7301882E-595.000016
GCGTCGA201.7301882E-595.000019
GCGTCAC201.7301882E-595.000017
TGTCGCG409.094947E-1295.000013
CGATACG201.7301882E-595.000011
Produced by FastQC (version 0.11.5)