FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118731
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAA9230.777387539901121No Hit
CGTCGTACGTTTCGACCTCGGTTAGAAGACTCATCTTTAAATATCTACCT1720.14486528370855126No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC1610.13560064347137646No Hit
CGTCGTACGCTGTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTTT1350.1137024029107815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA156.6031876E-495.08
GTCGCGT353.274181E-1095.06
CTCGTAC700.095.01
CGTATAG254.5950037E-795.01
CGAGTAG1000.095.01
CGTGAGC301.2223609E-895.01
CGCATAG156.6031876E-495.09
CTGACGT700.095.03
TGTGATA156.6031876E-495.05
AGAGTAC500.095.03
ACGATGT301.2223609E-895.05
GAGACGT254.5950037E-795.02
CTAGTCG353.274181E-1095.03
GTAGAGT353.274181E-1095.02
CGCTACA550.095.01
GTGTATA156.6031876E-495.02
CATACGA600.095.08
CTCGCAT156.6031876E-495.07
CGTGTAT156.6031876E-495.01
CGAGATG1200.095.01
Produced by FastQC (version 0.11.5)