FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12557126
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT220140.17531081554808003No Hit
CGACGCGTGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC152980.1218272397680807TruSeq Adapter, Index 5 (100% over 35bp)
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC152210.12121404213034097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT687200.094.291621
AGTACGA1041950.092.179237
GAGTACG1044250.091.994396
GTACGAT1046750.091.6026158
CGACTAT321200.091.402491
CGTGTCG245450.091.3278961
GTGTCGC243050.091.261992
CGCGCGT249450.090.9769444
CGTATAG647300.090.762061
TCGCGCG252250.090.6072853
TGTCGCG244850.090.3970953
CGCGTCA251000.090.170916
GTATGAG1075700.090.049222
GCGCGTC252500.089.917425
TATACTC715850.089.814327
GTGTATA720900.089.789542
ATGAGTA1074650.089.593564
GCGCACT245250.089.5929647
CGTAGTA154300.089.425731
CGCGCAC246200.089.42096