FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156034
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGTAGATATTTAAAGATGAGT6320.40503992719535487No Hit
CGAGCTCTATACGTGTAGTCTCCTAACCGAGGTCGAAACGTACGTTCTTT5940.38068626068677336No Hit
CGAGCTCTATACGTGTAGTCTCAGCAAAAGCAGGTAGATATTTAAAGATG5660.3627414537857134No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCTCAAAGCCGAGATCGCGCAG4790.3069843752002769No Hit
CGAGCTCTATACGTGTAGTCTCAGGCACTCCTTCCGTAGAAGGCCCTCTT4720.3024981734750119No Hit
CGAGCTCTATACGTGTAGTCTCTATGTTGACAAAATGACCATCGTCAACA4660.298652857710499No Hit
CGAGCTCTATACGTGTAGTCTCAGTAGAAACAAGGTAGTTTTTTACTCTA4580.2935257700244818No Hit
CGAGCTCTATACGTGTAGTCTCCGAAGAAATAAGACCCTTCATTACTCAT4130.26468590179063534No Hit
CGAGCTCTATACGTGTAGTCTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT4060.26019970006537035No Hit
CGAGCTCTATACGTGTAGTCTCAACCGATTAGAGACCTTGATACTACTAA3900.24994552469333609No Hit
CGAGCTCTATACGTGTAGTCTCCATCCCCATTTAGGGCATTTTGGATAAA3560.2281554020277632No Hit
CGAGCTCTATACGTGTAGTCTCAACCGAGGTCGAAACGTACGTTCTTTCT3250.20828793724444675No Hit
CGAGCTCTATACGTGTAGTCTCCATACCCATTTAGGGCATTTTGGATAAA2980.19098401630413883No Hit
CGAGCTCTATACGTGTAGTCTCAGCCGATTAGAGACCTTGATACTACTAA2700.17303920940307882No Hit
CGAGCTCTATACGTGTAGTCTCCGAGGGCGAAACGTACGTTCTTTCTATC2600.16663034979555738No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGAGTTTAAAATGAATCCAAA2510.16086237614878807No Hit
CGAGCTCTATACGTGTAGTCTCATGCCCATTTAGGGCATTTTGGATAAAG2400.1538126305805145No Hit
CGAGCTCTATACGTGTAGTCTCATTCCCATTTAGGGCATTTTGGATAAAG2320.14868554289449734No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCATTCCCATTTAGGGCATTTT2140.13714959560095877No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGGTGACAAAAACATAATGGA2080.13330427983644588No Hit
CGAGCTCTATACGTGTAGTCTCAGTCCCCATTCCCATTTAGGGCATTTTG2060.1320225079149416No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGCTTCTGTGGTCACTGTTCCC2050.13138162195418945No Hit
CGAGCTCTATACGTGTAGTCTCCGTGCCCAGTGAGCGAGGACTGCAGCGT1940.12433187638591589No Hit
CGAGCTCTATACGTGTAGTCTCGGCAGGGGAAAACAAAAGCAACAAAAAT1920.1230501044644116No Hit
CGAGCTCTATACGTGTAGTCTCATTCGCTTCTGGTAGGCCTGCAAATTTT1870.1198456746606509No Hit
CGAGCTCTATACGTGTAGTCTCATTCGCATTTAGGGCATTTTGGATAAAG1840.11792301677839445No Hit
CGAGCTCTATACGTGTAGTCTCCAGCCAGCAATGTTACATTTACCCAAAT1840.11792301677839445No Hit
CGAGCTCTATACGTGTAGTCTCCTTCCTCCCTCATCCCCACTTCACTGAA1760.11279592909237732No Hit
CGAGCTCTATACGTGTAGTCTCTGCGCGATCTCGGCTTTGAGGGGGCCTG1730.11087327121012087No Hit
CGAGCTCTATACGTGTAGTCTCAGCAAAAGCAGGGTGACAAAAACATAAT1720.11023238524936874No Hit
CGAGCTCTATACGTGTAGTCTCAGTAGCAACAAGGTAGTTTTTTACTCTA1670.10702795544560802No Hit
CGAGCTCTATACGTGTAGTCTCATCCCATTTAGGGCATTTTGGATAAAGC1640.10510529756335159No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGAGAAATAACGTTCCATGGGGCCA1640.10510529756335159No Hit
CGAGCTCTATACGTGTAGTCTCGCGTGATCAGTAGAAACAAGGTAGTTTT1610.10318263968109515No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGGTAGATAATCACTCAATGA1580.10125998179883872No Hit
CGAGCTCTATACGTGTAGTCTCCGAGGTCGAAACGTACGTTCTTTCTATC1570.10061909583808656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGC600.095.181121
AGCGCTA600.094.9980853
ATACGTT156.6076464E-494.9980859
GCGCTAT600.094.9980854
CTATGCG201.7392627E-594.998087
ATACGAG201.7392627E-594.998089
TATACGA201.7392627E-594.998088
CTGTACG353.274181E-1094.998077
TAGACGT353.274181E-1094.998078
GCTCTGT353.274181E-1094.998074
ATACGTG156150.092.807929
TATACGT156200.092.74788
CTATACG158150.092.02477
TCTATAC157550.091.9832156
CGAGCTC157600.091.9500351
GAGCTCT158250.091.846482
CTCTATA158150.091.754395
AGCTCTA164500.088.385743
GCTCTAT164950.088.000644
GCTATAC600.087.081586
Produced by FastQC (version 0.11.5)