FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44958
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACGACTACGCGCTCACTAACCGAGGTCGAAACGTACGTTCTTTCT4250.945326749410561No Hit
CGACGACGACTACGCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCT3440.7651585924640776No Hit
CGACGACGACTACGCGCTCACTAGACCAAAAGCAGCTTCTGTGGTCACTG3060.6806352595756039No Hit
CGACGACGACTACGCGCTCACTCAAAGCCGAGATCGCGCAGAGACTGGAA2370.527158681436007No Hit
CGACGACGACTACGCGCTCACTCGATCCAGCCATCTGTTCCATAGCCTTT2150.4782241202900485No Hit
CGACGACGACTACGCGCTCACTGGTGGGGAGCGCCAGGATGCCACAGAAA2120.47155122558832685No Hit
CGACGACGACTACGCGCTCACTGTTCCCATCCTGTTGTATATGAGGCCCA1670.37145780506250275No Hit
CGACGACGACTACGCGCTCACTAAGCTATTCAACTGGTGCACTTGCCAGT1670.37145780506250275No Hit
CGACGACGACTACGCGCTCACTGTGCCCAGTGAGCGAGGACTGCAGCGTA1350.3002802615774723No Hit
CGACGACGACTACGCGCTCACTAGGCAAAAGATAATAGGCCCTCTTTGCG1240.2758129810044931No Hit
CGACGACGACTACGCGCTCACTTGGGTAAATCAGACATATGTTAACATCA1150.25579429689932826No Hit
CGACGACGACTACGCGCTCACTTGAATCGCTGCATCTGCACTCCCATTCG1130.2513457004315139No Hit
CGACGACGACTACGCGCTCACTCCAACACCATGTCAAGCTTTCAGGTAGA1110.24689710396369946No Hit
CGACGACGACTACGCGCTCACTATTCGCTTCTGGTAGGCCTGCAAATTTT1070.23799991102807064No Hit
CGACGACGACTACGCGCTCACTGTAGAGACCCATTAGAGCACATCCAGAA1000.2224298233907202No Hit
CGACGACGACTACGCGCTCACTCGAAGCCGAGATCGCGCAGAGACTGGAA960.21353263045509144No Hit
CGACGACGACTACGCGCTCACTAGACGATACTGGACCACAACTGCCTGTC940.209084033987277No Hit
CGACGACGACTACGCGCTCACTCAGCAAAAGCAGGTAGATATTTAAAGAT940.209084033987277No Hit
CGACGACGACTACGCGCTCACTGTTTACACCACAAAAGGATATGCTGCTC860.1912896481160194No Hit
CGACGACGACTACGCGCTCACTGGAGCTAGGATGAGTCCCAATAGTTCTC850.18906534988211218No Hit
CGACGACGACTACGCGCTCACTAGTAGAGCCGGGAGACAAAATAACATTC760.16904666577694738No Hit
CGACGACGACTACGCGCTCACTAGGCACCAAACGATCATATGAACAAATG760.16904666577694738No Hit
CGACGACGACTACGCGCTCACTGGTGGTGAGCGCCAGGATGCCACAGAAA750.16682236754304017No Hit
CGACGACGACTACGCGCTCACTCAGCACCGTCTGGCCAAGACCAACCCAC720.16014947284131856No Hit
CGACGACGACTACGCGCTCACTAGAATCAGGATAACAGGAGCATTCCTCA660.14680368343787534No Hit
CGACGACGACTACGCGCTCACTTGGGAATCAAAATCAGATTGAAACATGC650.14457938520396815No Hit
CGACGACGACTACGCGCTCACTCAGCAAAAGCAGGGTGACAAAAACATAA650.14457938520396815No Hit
CGACGACGACTACGCGCTCACTCCCATTCGCTTCTGGTAGGCCTGCAAAT610.13568219226833936No Hit
CGACGACGACTACGCGCTCACTGGGGCCAAGGAGGTGTCACTAAGCTATT600.13345789403443215No Hit
CGACGACGACTACGCGCTCACTGGGGGACAAAAACATAATGGACTCCAAC600.13345789403443215No Hit
CGACGACGACTACGCGCTCACTACGCGTGATCAGTAGAAACAAGGTAGTT580.12900929756661772No Hit
CGACGACGACTACGCGCTCACTACGGCAAAGGCTATGGAACAGATGGCTG580.12900929756661772No Hit
CGACGACGACTACGCGCTCACTAAGACCACTGTGGACCATATGGCCATAA570.12678499933271054No Hit
CGACGACGACTACGCGCTCACTGGAGGTAGTGAAGGTCTCCCATTCTCAT560.12456070109880334No Hit
CGACGACGACTACGCGCTCACTGGGATGCTCTTCTAGGTATTTATTTCTT550.12233640286489612No Hit
CGACGACGACTACGCGCTCACTACGCGTGATCAGCAAAAGCAGGTAGATA530.11788780639708171No Hit
CGACGACGACTACGCGCTCACTGTTGACAAAATGACCATCGTCAACATCC530.11788780639708171No Hit
CGACGACGACTACGCGCTCACTGGGGGGGAGCGCCAGGATGCCACAGAAA480.10676631522754572No Hit
CGACGACGACTACGCGCTCACTAGACCCAAAGCAGCTTCTGTGGTCACTG480.10676631522754572No Hit
CGACGACGACTACGCGCTCACTGGGGGGCCAAGGAGGTGTCACTAAGCTA460.10231771875973131No Hit
CGACGACGACTACGCGCTCACTGGGGAGGGAACAATAGTTGGAGAAATTT460.10231771875973131No Hit
CGACGACGACTACGCGCTCACTACCACCAATCCACTAATCAGGCATGAAA460.10231771875973131No Hit
CGACGACGACTACGCGCTCACTCATGGACTCAGGCACTCCTTCCGTAGAA450.1000934205258241No Hit
CGACGACGACTACGCGCTCACTAAGCAAGATATCGTAGGAATAAATGAGT450.1000934205258241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACG46800.089.819518
ACTACGC46900.089.729269
GACGACT46750.089.712375
CGACTAC46750.089.712377
ACGACTA46650.089.701066
GACGACG49150.084.848522
ACGACGA49050.084.8278353
GAGGACG301.2880254E-463.3303682
AGGACGA301.2880254E-463.3303683
GACGGCT301.2880254E-463.3303685
GGCTACG301.2880254E-463.3303688
CGGCGAC250.00490348657.2520681
GCTACGC352.7597885E-454.2831769
ACGACGG352.7597885E-454.2831763
ACGGCTA352.7597885E-454.2831766
GGACGAC352.7597885E-454.2831764
CGTTCCA301.4773414E-647.49777682-83
CTCGAAC205.362797E-447.49777620-21
CTACAAG205.362797E-447.49777620-21
AACGTTC301.4773414E-647.49777680-81
Produced by FastQC (version 0.11.5)