FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92335
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAGCTACGTCGTGCACGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA4430.4797747333080631No Hit
CGTGAGCTACGTCGTGCACGTAGGGGCGGCAAGATTATTAGCGGTTTTGC3870.41912600855580223No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGAAATGAAGCAGGGGGCTGCGC3720.4028808144257324No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGAGAAGCTTTCAGTCTCGTACCA2890.31299074023934587No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGCTGCGCGCGGCGTAGAAAACTGC2480.26858720961715493No Hit
CGTGAGCTACGTCGTGCACGTAGACGGGGAAATGAAGCAGGGGGCTGCGC2040.22093464016895004No Hit
CGTGAGCTACGTCGTGCACGTAAGAGGGGGAACACGTACAATGCTTGTTT2020.2187686142849407No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGAGGAACACGTACAATGCTTGTTT1790.1938593166188336No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGGAAGCTTCGTGCTGGAGAAAA1790.1938593166188336No Hit
CGTGAGCTACGTCGTGCACGTAGTTGGCGGCAAGATTATTAGCGGTTTTG1770.19169329073482427No Hit
CGTGAGCTACGTCGTGCACGTAATGACCCCGCCACCCAAGATAAAGCGTT1770.19169329073482427No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGGGCAAAACCGCTAATAATCTTGC1620.17544809660475444No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGGGCTAACCCTAATGGCAAGGACT1600.1732820707207451No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGACAAAAACATAATGGACTCCAAC1580.1711160448367358No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAGATTATTAGCGGTTTTGC1570.17003303189473115No Hit
CGTGAGCTACGTCGTGCACGTAGAAAAGCGAAAGCATAGAAAGTGAGTGT1450.15703687659067528No Hit
CGTGAGCTACGTCGTGCACGTACCCCGCCACCCAAGATAAAGCGTTCAGT1430.15487085070666595No Hit
CGTGAGCTACGTCGTGCACGTAGGATGGGCAAAACCGCTAATAATCTTGC1410.15270482482265663No Hit
CGTGAGCTACGTCGTGCACGTACGCGTGATCAGCAAAAGCAGGTGGCACG1370.148372773054638No Hit
CGTGAGCTACGTCGTGCACGTAGGACGGGGAAATGAAGCAGGGGGCTGCG1270.13754264363459143No Hit
CGTGAGCTACGTCGTGCACGTACTCAAAATCAAATAATAGTTGTAAAGTG1260.1364596306925868No Hit
CGTGAGCTACGTCGTGCACGTAGGCGGGGAAATGAAGCAGGGGGCTGCGC1240.13429360480857747No Hit
CGTGAGCTACGTCGTGCACGTAGAGGCGGCAAGATTATTAGCGGTTTTGC1180.12779552715654952No Hit
CGTGAGCTACGTCGTGCACGTAGGGTGTGTGAGAGTGTGTGTGTATGTGC1170.12671251421454485No Hit
CGTGAGCTACGTCGTGCACGTAGAGGGGCCAAGGAGGTGTCACTAAGCTA1150.12454648833053554No Hit
CGTGAGCTACGTCGTGCACGTACAAGTGGCACACACTAGACCAAAAGCAG1150.12454648833053554No Hit
CGTGAGCTACGTCGTGCACGTAGGGGAGGCAAGAGAAGAACCCCGCACTC1120.12129744950452159No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAGAAGGGGATTGAACTCAG1120.12129744950452159No Hit
CGTGAGCTACGTCGTGCACGTAAACGGGGAAATGAAGCAGGGGGCTGCGC1110.12021443656251693No Hit
CGTGAGCTACGTCGTGCACGTATGTTGACAAAATGACCATCGTCAACATC1050.11371635891048897No Hit
CGTGAGCTACGTCGTGCACGTATTTGGGCATTAATTTCTTTACTTGTTGT1050.11371635891048897No Hit
CGTGAGCTACGTCGTGCACGTACGGCAAAGGCTATGGAACAGATGGCTGG1050.11371635891048897No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAAACCGCTAATAATCTTGC1010.10938430714247036No Hit
CGTGAGCTACGTCGTGCACGTATTGGGAAGAGTTGTCGTTGCTTCATGAC1000.10830129420046569No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGGAAATGAAGCAGGGGGCTGCG940.10180321654843776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT254.5879278E-794.996219
CTGCGTC201.7356513E-594.996217
GCTACGG201.7356513E-594.996216
TGCGTCG201.7356513E-594.996218
AACGTCG156.599538E-494.99628
CAACGTC156.599538E-494.99627
ACGTCGG301.2196324E-894.99629
AGCTCCG156.599538E-494.99625
GCGTCGT156.599538E-494.99629
GCTCCGT156.599538E-494.99626
TACGTCT156.599538E-494.99628
GCTACGT90900.094.943956
ACGTCGT90300.094.891019
TACGTCG90500.094.838758
CGTGAGC91350.094.627091
CTACGTC91050.094.422367
TGAGCTA92000.094.221793
AGCTACG92100.094.1194845
GTGAGCT92400.093.91672
GAGCTAC94750.091.436994
Produced by FastQC (version 0.11.5)