FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90546
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAGTCGCGAGTGTGAGCGCAGAGACTGGAAAGTGTCTTTGCAGGAA3920.43292911890089014No Hit
CGACGAGTCGCGAGTGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGT3380.3732909239502573No Hit
CGACGAGTCGCGAGTGTGAGCGCGGAGACTGGAAAGTGTCTTTGCAGGAA2610.28825127559472535No Hit
CGACGAGTCGCGAGTGTGAGCGCAGAGCCTGGAAAGTGTCTTTGCAGGAA2570.28383363152430807No Hit
CGACGAGTCGCGAGTGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAA2390.26395423320743044No Hit
CGACGAGTCGCGAGTGTGAGCGCAAAGCAGGTAGATATTTAAAGATGAGT1940.21425573741523646No Hit
CGACGAGTCGCGAGTGTGAGCGAAAAGCAGGTAGATATTTAAAGATGAGT1830.202107216221589No Hit
CGACGAGTCGCGAGTGTGAGCGTCTGGCTGTCAGTAAGTATGCTAGAGTC1780.19658516113356747No Hit
CGACGAGTCGCGAGTGTGAGCGCGCAGAGACTGGAAAGTGTCTTTGCAGG1760.19437633909835883No Hit
CGACGAGTCGCGAGTGTGAGCGATCAGCAAAAGCAGGTAGATATTTAAAG1570.17339252976387692No Hit
CGACGAGTCGCGAGTGTGAGCGCTGAGACTGGAAAGTGTCTTTGCAGGAA1560.17228811874627262No Hit
CGACGAGTCGCGAGTGTGAGCGTGATCAGTAGAAACAAGGTCGTTTTTAA1380.152408720429395No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGGAAGAATATCAACATCCACAGCAC1320.14578225432376912No Hit
CGACGAGTCGCGAGTGTGAGCGAAGACCACTGTGGACCATATGGCCATAA1260.13915578821814326No Hit
CGACGAGTCGCGAGTGTGAGCGAAAACATAATGGACTCCAACACCATGTC1260.13915578821814326No Hit
CGACGAGTCGCGAGTGTGAGCGCTACGCGTGATCAGTAGAAACAAGGTCG1230.13584255516533034No Hit
CGACGAGTCGCGAGTGTGAGCGCAGAGGCCATGGCCTCTGCTGCCTGTTC1100.12148521193647427No Hit
CGACGAGTCGCGAGTGTGAGCGGAGGGAAGAATATCAACATCCACAGCAC1080.11927638990126566No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGCAGAAATTTGAGGAAATAAGCTGA1020.11264992379563979No Hit
CGACGAGTCGCGAGTGTGAGCGAGTAGAAACAAGGTAGTTTTTTACTCTA1020.11264992379563979No Hit
CGACGAGTCGCGAGTGTGAGCGCTACGCGTGATCAGTAGAAACAAGGTAT1010.11154551277803547No Hit
CGACGAGTCGCGAGTGTGAGCGTGATCAGTAGAAACAAGGTAGTTTTTTA1000.11044110176043118No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGGCAGAAATTTGAGGAAATAAGCTG990.10933669074282686No Hit
CGACGAGTCGCGAGTGTGAGCGGTAGAAACAAGGTAGTTTTTTACTCTAG990.10933669074282686No Hit
CGACGAGTCGCGAGTGTGAGCGGGCAGCAGAGGCCATGGAGGTTGCTAAT990.10933669074282686No Hit
CGACGAGTCGCGAGTGTGAGCGGTCAGTAAGTATGCTAGAGTCCCGTTTT980.10823227972522254No Hit
CGACGAGTCGCGAGTGTGAGCGCTAAGACCACTGTGGACCATATGGCCAT970.10712786870761823No Hit
CGACGAGTCGCGAGTGTGAGCGGTAGGAACAAGGTAGTTTTTTACTCTAG960.10602345769001392No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGGGCCAAGGAGGTGTCACTAAGCTA950.1049190466724096No Hit
CGACGAGTCGCGAGTGTGAGCGCTACGCGTGATCAGCAAAAGCAGGTAAA920.10160581361959667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGC254.5894558E-794.98845
TCGCGGG254.5894558E-794.98848
ACGAGTG254.5894558E-794.98843
CCGCGAG156.601275E-494.9883968
GGGTCGC301.2201781E-894.9883965
AAGAGTC156.601275E-494.9883963
GACGGGT301.2201781E-894.9883962
AGTCGGG156.601275E-494.9883966
ACGGGTC301.2201781E-894.9883963
GACGAGC156.601275E-494.9883962
CGAGCCG156.601275E-494.9883964
CGCGGGT201.7361761E-594.9883969
GCCGCGA156.601275E-494.9883967
AGCCGCG156.601275E-494.9883966
TGAGTCG156.601275E-494.9883964
GTCGGGA156.601275E-494.9883967
TCGGGAG156.601275E-494.9883968
CGACGAG89650.093.974421
CGCGAGT90200.093.777359
GTCGCGA90400.093.7274867
Produced by FastQC (version 0.11.5)