FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8018
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGCGAGACAGTCGTGTCGAAAACATAATGGACTCCAACACCATGTC310.3866300823147917No Hit
CGCGAGCGAGACAGTCGTGTCGAACCGAGGTCGAAACGTACGTTCTTTCT260.32427039161885757No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGGACAAAAACATAATGGACTCCAAC250.3117984534796707No Hit
CGCGAGCGAGACAGTCGTGTCGTCAACATCCACAGCACTCTGCTGTTCCT230.28685457720129703No Hit
CGCGAGCGAGACAGTCGTGTCGCGCAGAGACTGGAAAGTGTCTTTGCAGG230.28685457720129703No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGAGGAATCCAGCGAGACACTTAGA210.26191070092292346No Hit
CGCGAGCGAGACAGTCGTGTCGCAGGGGACAAAAACATAATGGACTCCAA210.26191070092292346No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGACAAAAACATAATGGACTCCAACA180.22449488650536295No Hit
CGCGAGCGAGACAGTCGTGTCGCAGACCCGCACTCGCGAGATACTCACTA180.22449488650536295No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAGAGGAATCCAGCGAGACACTTAGA180.22449488650536295No Hit
CGCGAGCGAGACAGTCGTGTCGACAAAAACATAATGGACTCCAACACCAT150.18707907208780244No Hit
CGCGAGCGAGACAGTCGTGTCGCAGCCCCGCACTCGCGAGATACTCACTA140.1746071339486156No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAGGTAACGCGAAGTAGGTACAGATG140.1746071339486156No Hit
CGCGAGCGAGACAGTCGTGTCGATCAGCAAAAGCAGGTAGATATTTAAAG130.16213519580942878No Hit
CGCGAGCGAGACAGTCGTGTCGGAAGAGCACACGTCTGAACTCCAGTCAC120.14966325767024194Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CGCGAGCGAGACAGTCGTGTCGAGGGGGCCAAGGAGGTGTCACTAAGCTA120.14966325767024194No Hit
CGCGAGCGAGACAGTCGTGTCGCAGACAATGGATTGGGTGATGCCCCATT120.14966325767024194No Hit
CGCGAGCGAGACAGTCGTGTCGCGTAGAGACTGGAAAGTGTCTTTGCAGG120.14966325767024194No Hit
CGCGAGCGAGACAGTCGTGTCGAAACGCACGTTCTTTCTATCATCCCGTC120.14966325767024194No Hit
CGCGAGCGAGACAGTCGTGTCGGAGGGGGGAAAATGGACGAAGGACAAGG120.14966325767024194No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGAACATAGTACTGAAAGCGAACTT110.13719131953105512No Hit
CGCGAGCGAGACAGTCGTGTCGACGGACAGAAATTTGAGGAAACAAGGGT110.13719131953105512No Hit
CGCGAGCGAGACAGTCGTGTCGAAGACCACTGTGGACCATATGGCCATAA110.13719131953105512No Hit
CGCGAGCGAGACAGTCGTGTCGCGAGATCGGAAGAGCACACGTCTGAACT110.13719131953105512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGAGCGAGACAGTCGTGTCGGAAAGGGGAATCCAGCGAGACACTTAGA100.1247193813918683No Hit
CGCGAGCGAGACAGTCGTGTCGATCAGCAAAAGCAGGGTGACAAAAACAT100.1247193813918683No Hit
CGCGAGCGAGACAGTCGTGTCGCGTCCCGCACTCGCGAGATACTCACTAA100.1247193813918683No Hit
CGCGAGCGAGACAGTCGTGTCGCAGAGACTGGAAAGTGTCTTTGCAGGAA100.1247193813918683No Hit
CGCGAGCGAGACAGTCGTGTCGGGCAAGAGAAGAACCCCGCACTCAGAAT90.11224744325268148No Hit
CGCGAGCGAGACAGTCGTGTCGGGCAGGGCAAATATATTCAATATGGAGA90.11224744325268148No Hit
CGCGAGCGAGACAGTCGTGTCGACAAAATGACCATCGTCAACATCCACAG90.11224744325268148No Hit
CGCGAGCGAGACAGTCGTGTCGGCACAGAGAAAGCTCTTATCTCTTGTTC90.11224744325268148No Hit
CGCGAGCGAGACAGTCGTGTCGAAACAAGGAAGTTTTTTACTCTAGCTCT90.11224744325268148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAG7850.094.993754
CGAGCGA7800.094.9937443
AGACAGT7900.094.9937449
CGCGAGC7800.094.978431
GCGAGCG7850.094.3886952
GAGACAG8000.093.806338
CGAGACA8050.093.223687
AGCGAGA8000.093.212625
GCGAGAC8000.093.212626
AGGGGAA205.231201E-447.49687626-27
GTGTCGC1550.047.49687616-17
GTCGCGG252.7040822E-547.49687618-19
TCGGTAG205.231201E-447.49687620-21
TCGAAAA205.231201E-447.49687620-21
GTCGACA357.288327E-847.49687618-19
TCGGAGA205.231201E-447.49687620-21
TCGGACA252.7040822E-547.49687620-21
CCGCAAG205.231201E-447.49687692-93
CCTCTTT205.231201E-447.49687680-81
GGGGGGC205.231201E-447.49687622-23
Produced by FastQC (version 0.11.5)