FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100024
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTACACACTACGTAGCAAGGCGATCTTCCAGGAGCTAATGGTAGT7990.7988082860113573No Hit
CGAGAGTACACACTACGTAGCAACCTCCATGGCCTCTGCTGCCTGTTCAC3740.37391026153723106No Hit
CGAGAGTACACACTACGTAGCAAGAAGCTCAAAAGAGAAATAACGTTCCA2280.22794529312964887No Hit
CGAGAGTACACACTACGTAGCAGGAGCTAATGGTAGTGCCACCATCCAGG1530.15296328881068544No Hit
CGAGAGTACACACTACGTAGCACCCCAAGATCGAAGGTTCCAGGTTCCAG1360.1359673678317204No Hit
CGAGAGTACACACTACGTAGCAAGCCCATACACACAAGCAGGCAGGCAGG1320.13196832760137567No Hit
CGAGAGTACACACTACGTAGCACGGATATAAAGAATCAGGCCAAACCTAA1190.11897144685275535No Hit
CGAGAGTACACACTACGTAGCATGGGGCCAAGGAGGTGTCACTAAGCTAT1180.11797168679516916No Hit
CGAGAGTACACACTACGTAGCACTGCCCAATTAGCACATTAGCTTTCTCT1170.11697192673758298No Hit
CGAGAGTACACACTACGTAGCAATTCGACACTAATTGATGGCCATCCGAA1160.1159721666799968No Hit
CGAGAGTACACACTACGTAGCAAGGAGCTTGCTTCTGTAGTGCTGTGTTG1150.11497240662241062No Hit
CGAGAGTACACACTACGTAGCAAAGGGGAGAAGTTTTATGTTCATTCCTT1120.1119731264496521No Hit
CGAGAGTACACACTACGTAGCAAGGCCCTCTTTTCAAACCGTATTTAAAG1120.1119731264496521No Hit
CGAGAGTACACACTACGTAGCACACGTCTGAACTCCAGTCACACAGTGAT1090.10897384627689354TruSeq Adapter, Index 5 (100% over 32bp)
CGAGAGTACACACTACGTAGCACTCCCATTCGCTTCTGGTAGGCCTGCAA1090.10897384627689354No Hit
CGAGAGTACACACTACGTAGCACTGGAGCTAGGATGAGTCCCAATAGTTC1080.10797408621930737No Hit
CGAGAGTACACACTACGTAGCAATGCCCAATTAGCACATTAGCTTTCTCT1010.10097576581620411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGT201.7151395E-595.2315751
CGAGAGT95150.095.03141
ACACCCT254.5915294E-794.993518
GTACACC201.7366101E-594.99356
GAGTACG201.7366101E-594.99354
AGAGTAG301.2210876E-894.99353
TACGCAC201.7366101E-594.99357
TAGTACA156.6019123E-494.99354
GTACGCA201.7366101E-594.99356
GAGAGTA95750.094.745472
GAGTACA95600.094.745094
AGAGTAC95500.094.744823
CACACTA96900.094.552359
AGTACAC96350.093.76115
ACACACT97700.093.632288
GTACACA97100.093.526046
TACACAC97400.093.140447
AGGGTAC600.087.077383
GAGGGTA600.087.077382
TACATAC353.5592166E-881.4237
Produced by FastQC (version 0.11.5)