FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36105
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCATAGTGTACGTCGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTT2250.6231823847112589No Hit
CGCTCATAGTGTACGTCGACTCAGGCCAAACCTAACAAGGCTAGTCTTAC1440.39883672621520566No Hit
CGCTCATAGTGTACGTCGACTCAGGCCCTCCTTCCGTAGAAGGCCCTCTT1380.38221852928957206No Hit
CGCTCATAGTGTACGTCGACTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT1200.3323639385126714No Hit
CGCTCATAGTGTACGTCGACTCCAGAGCAGAAATGAAAAGTGGCGAGAGC890.24650325439689794No Hit
CGCTCATAGTGTACGTCGACTCAGTAGAAACAAGGTAGTTTTTTACTCTA880.24373355490929235No Hit
CGCTCATAGTGTACGTCGACTCGAGGCCAAGGGTGTTGCCTCTTCCTTTT730.2021880625952084No Hit
CGCTCATAGTGTACGTCGACTCTGGTTGTTAGTCCATTGTAATACTTTGC700.19387896413239164No Hit
CGCTCATAGTGTACGTCGACTCCACCCCAGATGTGCCTTCATCTGGATCT700.19387896413239164No Hit
CGCTCATAGTGTACGTCGACTCAGGCTCTCCTTCCGTAGAAGGCCCTCTT700.19387896413239164No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGCGAATGGGAGTGCAGATGCAGC670.18556986566957487No Hit
CGCTCATAGTGTACGTCGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGA650.18003046669436365No Hit
CGCTCATAGTGTACGTCGACTCCAGTCACACAGTGATCTCGTATGCCGTC570.1578728707935189TruSeq Adapter, Index 5 (96% over 33bp)
CGCTCATAGTGTACGTCGACTCCGGAAGCGAATGGGAGTGCAGATGCAGC520.14402437335549093No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGGGAATGGGAGTGCAGATGCAGC490.13571527489267415No Hit
CGCTCATAGTGTACGTCGACTCCCGCTATATCCTGACCACTCATTTATTC460.12740617642985735No Hit
CGCTCATAGTGTACGTCGACTCAGAAGCGAATGGGAGTGCAGATGCAGCG450.12463647694225177No Hit
CGCTCATAGTGTACGTCGACTCGAGAAGCGAATGGGAGTGCAGATGCAGC440.12186677745464618No Hit
CGCTCATAGTGTACGTCGACTCCAGCGCAAAATGCCATAAGCACCACATT440.12186677745464618No Hit
CGCTCATAGTGTACGTCGACTCCAACGCCATGTCAAGCTTTCAGGTAGAC440.12186677745464618No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGCAAATGGGAGTGCAGATGCAGC430.11909707796704057No Hit
CGCTCATAGTGTACGTCGACTCATTCCCATTTAGGGCATTTTGGATAAAG430.11909707796704057No Hit
CGCTCATAGTGTACGTCGACTCAGGAAACAGGTTTGATCTGATTAAATAC420.11632737847943497No Hit
CGCTCATAGTGTACGTCGACTCTCTGGTTGTTAGTCCATTGTAATACTTT420.11632737847943497No Hit
CGCTCATAGTGTACGTCGACTCCCGAAGCGAATGGGAGTGCAGATGCAGC410.11355767899182939No Hit
CGCTCATAGTGTACGTCGACTCAGTAGAAACAAGGTCGTTTTTAAACAAT400.1107879795042238No Hit
CGCTCATAGTGTACGTCGACTCAGCACCGTCTGGCCAAGACCAACCCACA380.10524858052901259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAT34850.095.3866351
CTCATAG35450.094.860483
ATAGTGT35400.094.860286
TCATAGT35350.094.860094
GCTCATA35150.094.859332
GTGTACG35650.094.7289
TAGTGTA35600.094.7276157
AGTGTAC35550.094.727258
CATAGTG35450.094.7264945
CACTCAT408.4841304E-871.642761
ACTCATA504.1289786E-756.9966742
CTCACCC301.473376E-647.49723420-21
GCCGAAA301.473376E-647.49723424-25
CTCGGGA600.047.49723420-21
CTCGCAA301.473376E-647.49723420-21
TAAGGTG301.473376E-647.49723474-75
ACTCCGG1200.047.49723418-19
ACTCCGC600.047.49723418-19
GACGATC600.047.49723494-95
AGGTGGT301.473376E-647.49723476-77
Produced by FastQC (version 0.11.5)