FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130708
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACTACGACTGCACGTACAAGGCACCAAACGATCATATGAACAAATG4790.36646570982648347No Hit
CGAGACTACGACTGCACGTACAAAAACATAATGGACTCCAACACCATGTC4760.36417051748936563No Hit
CGAGACTACGACTGCACGTACAATGAACTGGCGACAGTTGAATAGATCGC4710.36034519692750255No Hit
CGAGACTACGACTGCACGTACAACACCATGTCAAGCTTTCAGGTAGACTG4180.31979679897175384No Hit
CGAGACTACGACTGCACGTACACGAAGAAATAAGACCCTTCATTACTCAT4060.31061602962328244No Hit
CGAGACTACGACTGCACGTACAATGACCGAAATTTCTGGAGGGGTGAAAA3800.2907243627015944No Hit
CGAGACTACGACTGCACGTACACAGAGCAGAAATGAAAAGTGGCGAGAGC3370.25782660586957185No Hit
CGAGACTACGACTGCACGTACAAGAAGCTCAAAAGAGAAATAACGTTCCA3080.235639746610766No Hit
CGAGACTACGACTGCACGTACAGAGGGGGGAAAATGGACGAAGGACAAGG3030.2318144260489029No Hit
CGAGACTACGACTGCACGTACAATGCCAAGGAAATTGGAAACGGCTGCTT2950.225693913149922No Hit
CGAGACTACGACTGCACGTACACGCCCCTGGAAGGACAAATCTTCTGGCT2940.22492884903754937No Hit
CGAGACTACGACTGCACGTACAGAAAGCACCATCCTCTCTATTGTTATGC2740.209627566790097No Hit
CGAGACTACGACTGCACGTACACCACAAAAGGATATGCTGCTCCCGCTAG2640.20197692566637082No Hit
CGAGACTACGACTGCACGTACAGGGGGGAAAATGGACGAAGGACAAGGGT2630.2012118615539982No Hit
CGAGACTACGACTGCACGTACAAGCCCATACACACAAGCAGGCAGGCAGG2580.19738654099213512No Hit
CGAGACTACGACTGCACGTACACCGCCCCTGGAAGGACAAATCTTCTGGC2480.18973589986840897No Hit
CGAGACTACGACTGCACGTACACCAAGCAGGCAGGCAGGATTTATGTGCA2300.17596474584570188No Hit
CGAGACTACGACTGCACGTACAATGAGCTGGCGACAGTTGAATAGATCGC2260.17290448939621142No Hit
CGAGACTACGACTGCACGTACAGATGCAATTGTCGTTCTAAGTGTCTCGC2230.17060929705909356No Hit
CGAGACTACGACTGCACGTACACACAGCAGGCAGGCAGGATTTATGTGCA2200.1683141047219757No Hit
CGAGACTACGACTGCACGTACAAGGCCCTCTTTTCAAACCGTATTTAAAG2180.16678397649723045No Hit
CGAGACTACGACTGCACGTACACTTCTCAATAAAGCAAGATATCGTAGGA2150.16448878416011262No Hit
CGAGACTACGACTGCACGTACAATACCCTGGAACTAAGAAGCAGATACTG2130.16295865593536737No Hit
CGAGACTACGACTGCACGTACAAGCACCACATTCCCTTATACTGGAGATC1940.1484224378002877No Hit
CGAGACTACGACTGCACGTACACATCCAGAAACTGATTGCCCCCAGGGAG1910.14612724546316982No Hit
CGAGACTACGACTGCACGTACAAGAAGCAGATACTGGGCCATAAGGACCA1820.13924166845181626No Hit
CGAGACTACGACTGCACGTACACCGCGCTACCTTTCTGACATGACCCTCG1750.13388621966520795No Hit
CGAGACTACGACTGCACGTACACAAATCCTAAAATTCCCTTAGTCAGAGG1720.13159102732809008No Hit
CGAGACTACGACTGCACGTACACTGCGCGCTACCTTTCTGACATGACCCT1590.12164519386724608No Hit
CGAGACTACGACTGCACGTACAATGCGATAACACGTGCATGGAAAGTGTC1540.117819873305383No Hit
CGAGACTACGACTGCACGTACAATTAGCACATTAGCTTTCTCTCCTTTTG1490.11399455274351991No Hit
CGAGACTACGACTGCACGTACAATTCGACACTAATTGATGGCCATCCGAA1480.11322948863114729No Hit
CGAGACTACGACTGCACGTACAGGGGGGCAAAAACATAATGGACTCCAAC1480.11322948863114729No Hit
CGAGACTACGACTGCACGTACAGAAACGAGAAAGCTCTTATCTCTTGTTC1460.11169936040640205No Hit
CGAGACTACGACTGCACGTACAATTCGCTTCTGGTAGGCCTGCAAATTTT1440.11016923218165682No Hit
CGAGACTACGACTGCACGTACAATAACTTCTCAATAAAGCAAGATATCGT1430.1094041680692842No Hit
CGAGACTACGACTGCACGTACAAGAAACTGATTGCCCCCAGGGAGACTAC1400.10710897573216636No Hit
CGAGACTACGACTGCACGTACAAAAGCAGAAAGCACCATCCTCTCTATTG1360.10404871928267588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT750.095.108011
CGAGAGT450.095.108011
CTAGACT156.574918E-495.1081
CGTGACT301.2138116E-895.1081
CGAGACC156.574918E-495.1081
AGACTTC254.5979505E-794.9988563
CTTCGAC254.5979505E-794.9988566
ACGCCTG201.7381311E-594.9988568
GAGTACG450.094.9988564
AAGACTA750.094.9988562
GAGAGTA450.094.9988562
TACGGCT254.5979505E-794.9988567
GACTTCG254.5979505E-794.9988564
CGCCTGC201.7381311E-594.9988569
ACTAGGA409.094947E-1294.9988565
TACGCCT201.7381311E-594.9988567
TACGAGT254.5979505E-794.9988567
CTACGCC254.5979505E-794.9988566
CTACGAG254.5979505E-794.9988566
TTACGAC201.7381311E-594.9988566
Produced by FastQC (version 0.11.5)