FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173593
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGTCTCGACGTGTGTGTAGTAGCCATTTGTCTGTGAGACCGATGC8470.4879229001169402No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGGTGTT6930.3992096455502238No Hit
CGTGTCGTCTCGACGTGTGTGTAGTCCAAAGTTCTTTCATTTTCCAATAG6370.366950280253236No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGAGTTTAAAATGAATCCAAA6220.3583093788344No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGAGTTTAAAATGAATC6000.3456360567534405No Hit
CGTGTCGTCTCGACGTGTGTGTAGAAGCTTTTTGCTCCAGCATGAGGACA5400.3110724510780965No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGGTGACAAAAACATAATGGA5030.289758227578301No Hit
CGTGTCGTCTCGACGTGTGTGTATCAGCAAAAGCAGGAGTTTAAAATGAA4810.2770849054973415No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGGTGACAAAAACATAA4470.2574988622813132No Hit
CGTGTCGTCTCGACGTGTGTGTATCAGCAAAAGCAGGGTGACAAAAACAT4130.2379128190652849No Hit
CGTGTCGTCTCGACGTGTGTGTTCGGGTCCCCATTCCCATTTAGGGCATT3900.22466343688973636No Hit
CGTGTCGTCTCGACGTGTGTGTGGCAGGGGAAAACAAAAGCAACAAAAAT3870.22293525660596913No Hit
CGTGTCGTCTCGACGTGTGTGTAGGACCATGAGCTTGCTGTGGAGAGTGA3670.21141405471418778No Hit
CGTGTCGTCTCGACGTGTGTGTCTGACAAGTTGTATTGCAATCGTGGACT3590.20680557395747526No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGTAGATATTTAAAGATGAGT3390.1952843720656939No Hit
CGTGTCGTCTCGACGTGTGTGTCCCAGTCCATCCGTTCGGATCCCAAATC3320.19125195140357043No Hit
CGTGTCGTCTCGACGTGTGTGTCTGACCAGTTGTATTGCAATCGTGGACT3220.18549135045767973No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGTAGATATTTAAAGAT3190.18376317017391255No Hit
CGTGTCGTCTCGACGTGTGTGTGAGGGGGGAAAATGGACGAAGGACAAGG3130.18030680960637813No Hit
CGTGTCGTCTCGACGTGTGTGTTTTTTATCATTAAATAAGCTGAAACGAG3120.17973074951178905No Hit
CGTGTCGTCTCGACGTGTGTGTCTGCAAAAGCAGGAGTTTAAAATGAATC3070.17685044903884373No Hit
CGTGTCGTCTCGACGTGTGTGTAGCAGGGGAAAACAAAAGCAACAAAAAT3030.17454620866048748No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGCAAAAGCAGGGTGACA3020.1739701485658984No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGTAGTT3020.1739701485658984No Hit
CGTGTCGTCTCGACGTGTGTGTTGTTTTCATAAGTAATGACGCTTTGATT3000.17281802837672025No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGCAAAAGCAGGAGTTTA2990.17224196828213117No Hit
CGTGTCGTCTCGACGTGTGTGTAGGCCAAAGTTCTTTCATTTTCCAATAG2920.16820954762000773No Hit
CGTGTCGTCTCGACGTGTGTGTAGCGGGGGAAAACAAAAGCAACAAAAAT2870.16532924714706237No Hit
CGTGTCGTCTCGACGTGTGTGTTTTCATAAGTAATGACGCTTTGATTGCA2740.1578404659174045No Hit
CGTGTCGTCTCGACGTGTGTGTTACTGTTACATTCTTTTCTAGTACTGTG2640.15207986497151382No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGAGTTT2600.14977562459315755No Hit
CGTGTCGTCTCGACGTGTGTGTGCAGGGGAAAACAAAAGCAACAAAAATG2300.13249382175548552No Hit
CGTGTCGTCTCGACGTGTGTGTGGGGAGCGCCAGGATGCCACAGAAATCA2280.1313417015663074No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGGTAGATAATCACTCA2240.12903746118795112No Hit
CGTGTCGTCTCGACGTGTGTGTAAGACCTGCTGTTGCATCTTCGCCATTG2240.12903746118795112No Hit
CGTGTCGTCTCGACGTGTGTGTAAGTATGCTAGAGTCCCGTTTTCGTTTC2210.12730928090418392No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGGTAGATAATCACTCAATGA2180.12558110062041672No Hit
CGTGTCGTCTCGACGTGTGTGTTTATAGCACCCTTGGGTGTCTGACAAGT2170.12500504052582764No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGGTATT2110.12154867995829324No Hit
CGTGTCGTCTCGACGTGTGTGTAGGACCAAAGAAGGAAGACGGAAAACAA2040.11751625929616978No Hit
CGTGTCGTCTCGACGTGTGTGTCGGCAAAAGCAGGAGTTTAAAATGAATC2030.1169401992015807No Hit
CGTGTCGTCTCGACGTGTGTGTCTGCAAAAGCAGGGTGACAAAAACATAA1980.11405989872863537No Hit
CGTGTCGTCTCGACGTGTGTGTGAGACCGATGCTGTGAATCAGCAATCTG1900.10945141797192282No Hit
CGTGTCGTCTCGACGTGTGTGTGGGGAGGGAACTTCACCAATAGGACAGC1840.10599505740438843No Hit
CGTGTCGTCTCGACGTGTGTGTATCAGCAAAAGCAGGTAGATATTTAAAG1820.1048429372152103No Hit
CGTGTCGTCTCGACGTGTGTGTCGATTCGAGCCATGCCAGTTATCCCTGC1810.10426687712062123No Hit
CGTGTCGTCTCGACGTGTGTGTAGTCCCAAGTTCTTTCATTTTCCAATAG1790.10311475693144309No Hit
CGTGTCGTCTCGACGTGTGTGTTGTTCGGGTCCCCATTCCCATTTAGGGC1760.10138657664767589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG156.610852E-494.991079
GTCGACG301.22654455E-894.991079
CTCAACG156.610852E-494.991079
CGTCTTG201.7404545E-594.991076
TTCGTCT201.7404545E-594.991074
CGTCTAG156.610852E-494.991076
GTCTAGA156.610852E-494.991077
TCTAGAC156.610852E-494.991078
CGCCTCG156.610852E-494.991076
ACTCGAC156.610852E-494.991078
CTCGATG450.094.991079
GTCTTGA201.7404545E-594.991077
CGACTCG156.610852E-494.991076
TCGACTC156.610852E-494.991075
TATCGAC156.610852E-494.991078
CCCGACG156.610852E-494.991079
GTCGCGA254.6066089E-794.9910667
CGTCGCG254.6066089E-794.9910666
TCGCGAC254.6066089E-794.9910668
TCTCGAC171300.094.353368
Produced by FastQC (version 0.11.5)