FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138858
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGACGATCGACGCGCTGAAACGAGAAAGCTCTTATCTCTTGTTCT9650.6949545578936756No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGAGTTTAAAATGAA3330.23981333448558959No Hit
CGAGTAGACGATCGACGCGCTGCATCTGCACTCCCATTCGCTTCTGGTAG3170.22829077186766336No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGTAGATATTTAAAG2750.19804404499560702No Hit
CGAGTAGACGATCGACGCGCTGGGGGGGGGAAAATGGACGAAGGACAAGG2600.18724164254130118No Hit
CGAGTAGACGATCGACGCGCTGATGAGAGAAGAAATAAATACCTAGAAGA2530.18220052139595846No Hit
CGAGTAGACGATCGACGCGCTGACAAAATGACCATCGTCAACATCCACAG2380.1713981189416526No Hit
CGAGTAGACGATCGACGCGCTGTGGGGAGCGCCAGGATGCCACAGAAATC2190.15771507583286523No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGGTGACAAAAACAT2180.15699491566924484No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGAGTTTAAAATGAA2160.15555459534200405No Hit
CGAGTAGACGATCGACGCGCTGGGGGGAGCGCCAGGATGCCACAGAAATC2070.14907315386942058No Hit
CGAGTAGACGATCGACGCGCTGCACAGATTGAAAGCGACAGAGAATAGTT2030.146192513214939No Hit
CGAGTAGACGATCGACGCGCTGAACAGCAAAAGCAGGAGTTTAAAATGAA1940.1397110717423555No Hit
CGAGTAGACGATCGACGCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTC1940.1397110717423555No Hit
CGAGTAGACGATCGACGCGCTGAGCAGAAATGAAAAGTGGCGAGAGCAAT1670.12026674732460499No Hit
CGAGTAGACGATCGACGCGCTGGAGGGGGGAAAATGGACGAAGGACAAGG1660.11954658716098461No Hit
CGAGTAGACGATCGACGCGCTGAAGAGCACACAGAATGCCATTGACAAGA1640.11810626683374383No Hit
CGAGTAGACGATCGACGCGCTGAAAACAGAATGGTGCTGGCTAGCACTAC1580.11378530585202148No Hit
CGAGTAGACGATCGACGCGCTGGGGGGGGAAAATGGACGAAGGACAAGGG1560.11234498552478071No Hit
CGAGTAGACGATCGACGCGCTGATAAGCAAAAGCAGGAGTTTAAAATGAA1540.11090466519753993No Hit
CGAGTAGACGATCGACGCGCTGAGGCCATGGAGGTTGCTAATCAGACTAG1510.10874418470667875No Hit
CGAGTAGACGATCGACGCGCTGATGCAATTGTCATTCTAAGTGTCTCGCT1490.107303864379438No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGTAGATATTTAAAG1480.1065837042158176No Hit
CGAGTAGACGATCGACGCGCTGGTGGGAGCGCCAGGATGCCACAGAAATC1480.1065837042158176No Hit
CGAGTAGACGATCGACGCGCTGAACAGAGGTTATAAGAATGATGGAAAGT1470.10586354405219721No Hit
CGAGTAGACGATCGACGCGCTGAGCAGCAAAAGCAGGAGTTTAAAATGAA1430.10298290339771565No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGGTGACAAAAACAT1420.10226274323409526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG201.7114346E-595.299491
CGAATAG301.1974407E-895.299491
CGTGTAG201.7114346E-595.299491
CGAGTAG135850.095.1591951
AGACTAT156.608159E-494.990656
ACTATCG156.608159E-494.990658
GTAGAGG156.608159E-494.990654
CGATCGC156.608159E-494.990659
TGATCGA254.601843E-794.990649
GACGGTC254.601843E-794.990647
ACGAGCG254.601843E-794.990648
ACGTTCG201.739262E-594.990638
TAGACGT201.739262E-594.990635
GTAGGCG409.094947E-1294.990634
CGTTCGA201.739262E-594.990639
AGACGTT201.739262E-594.990636
CGCTCGA201.739262E-594.990639
ACGCTCG201.739262E-594.990638
GCGATCG409.094947E-1294.990638
TAGGCGA409.094947E-1294.990635
Produced by FastQC (version 0.11.5)