FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131424
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT16371.2455868030192354No Hit
CGAGATGACGAGACGCACGACGAAAACATAATGGACTCCAACACCATGTC5460.4154492330168006No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATTGCATCTGTACCTA5390.41012296079863647No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGGTGACAAAAACAT5190.3949050401753105No Hit
CGAGATGACGAGACGCACGACGCAAAGAGGGCCTATTATCTTTTGCCTAG5030.38273070367664963No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGTAGATATTTAAAG4660.3545775505234965No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGTAGTTTTT4430.3370769418066715No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATCGCACGCAAAGAGG4410.3355551497443389No Hit
CGAGATGACGAGACGCACGACGAATAGCATAACAATAGAGAGGATGGTGC4150.3157718529340151No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGAGTTTAAAATGAA4000.30435841246652057No Hit
CGAGATGACGAGACGCACGACGATAAGACAGGCAGTTGTGGTCCAGTATC3770.28685780374969566No Hit
CGAGATGACGAGACGCACGACGATCATATGAACAAATGGAGACTGGTGGG3510.2670745069393718No Hit
CGAGATGACGAGACGCACGACGACACCATGTCAAGCTTTCAGGTAGACTG3210.24424762600438274No Hit
CGAGATGACGAGACGCACGACGGAGGGGGGAAAATGAGGTCTTCAATCTC2920.22218164110056005No Hit
CGAGATGACGAGACGCACGACGCAGAGACTGGAAAGTGTCTTTGCAGGAA2850.2168553688823959No Hit
CGAGATGACGAGACGCACGACGAAGAGCATCCCAGTGCTGGGAAGGACCC2580.19631117604090578No Hit
CGAGATGACGAGACGCACGACGAAAAGGGAAAGATAGTCAAATCAGTCGA2450.18641952763574385No Hit
CGAGATGACGAGACGCACGACGAAGAAATAAGACCCTTCATTACTCATGT2430.18489773557341124No Hit
CGAGATGACGAGACGCACGACGGCAAAGGCTATGGAACAGATGGCTGGAT2140.16283175066958852No Hit
CGAGATGACGAGACGCACGACGATTCGAGCCATGCCAGTTATCCCTGCAC2050.1559836863890918No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGCAAAAGCAGGTAGATATTT2020.1537009982955929No Hit
CGAGATGACGAGACGCACGACGGAGGGGGGAAAATGGACGAAGGACAAGG2020.1537009982955929No Hit
CGAGATGACGAGACGCACGACGCTTCGCGTTACCTTTCTGACATGACCCT2000.15217920623326028No Hit
CGAGATGACGAGACGCACGACGGAAAAGGGAAAGATAGTCAAATCAGTCG2000.15217920623326028No Hit
CGAGATGACGAGACGCACGACGATCAGATGAACAAATGGAGACTGGTGGG1990.15141831020209398No Hit
CGAGATGACGAGACGCACGACGATCACATGAACAAATGGAGACTGGTGGG1960.1491356221085951No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTATTTTT1940.14761383004626247No Hit
CGAGATGACGAGACGCACGACGATTCAAATGTGAAGAACTTGTATGAAAA1920.1460920379839299No Hit
CGAGATGACGAGACGCACGACGAAGAGCACACAGAATGCCATTGACAAGA1810.13772218164110056No Hit
CGAGATGACGAGACGCACGACGAATCCAGAAAGCACCATCCTCTCTATTG1720.13087411736060384No Hit
CGAGATGACGAGACGCACGACGAAGCGAATGGGAGTGCAGATGCAGCGAT1710.13011322132943753No Hit
CGAGATGACGAGACGCACGACGCAGAGCAGAAATGAAAAGTGGCGAGAGC1640.12478694911127344No Hit
CGAGATGACGAGACGCACGACGATCAAATGAACAAATGGAGACTGGTGGG1620.12326515704894082No Hit
CGAGATGACGAGACGCACGACGAAAAGGGGAAGATAGTCAAATCAGTCGA1590.12098246895544194No Hit
CGAGATGACGAGACGCACGACGATTCACATGTGAAGAACTTGTATGAAAA1580.12022157292427563No Hit
CGAGATGACGAGACGCACGACGATTATATGAACAAATGGAGACTGGTGGG1570.11946067689310932No Hit
CGAGATGACGAGACGCACGACGGGGGGCAGAAATTTGAGGAAATAAGGTG1570.11946067689310932No Hit
CGAGATGACGAGACGCACGACGAGCAGGGGAAAACAAAAGCAACAAAAAT1530.11641709276844411No Hit
CGAGATGACGAGACGCACGACGATTCGAATGTGAAGAACTTGTATGAAAA1480.11261261261261261No Hit
CGAGATGACGAGACGCACGACGATTCGCTTCTGGTAGGCCTGCAAATTTT1460.11109082055028002No Hit
CGAGATGACGAGACGCACGACGGGTGGGGAGCGCCAGGATGCCACAGAAA1440.1095690284879474No Hit
CGAGATGACGAGACGCACGACGAACAGAGGTTATAAGAATGATGGAAAGT1410.1072863403944485No Hit
CGAGATGACGAGACGCACGACGGTGGGGAGCGCCAGGATGCCACAGAAAT1410.1072863403944485No Hit
CGAGATGACGAGACGCACGACGATCAGTAATCCACAATATCAGGTGCAAG1380.1050036523009496No Hit
CGAGATGACGAGACGCACGACGCCCCCAGGGAGACTACCAGTACCAATGA1380.1050036523009496No Hit
CGAGATGACGAGACGCACGACGATAAATACATTTGAAAAAAAGACGATCA1360.10348186023861698No Hit
CGAGATGACGAGACGCACGACGAGAAAGCTCTTATCTCTTGTTCTACTTC1350.1027209642074507No Hit
CGAGATGACGAGACGCACGACGGGGGGGGCAATAGTTGGAGAAATTTCAC1340.10196006817628439No Hit
CGAGATGACGAGACGCACGACGGGGGACAGAAATTTGAGGAAATAAGGTG1330.1011991721451181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGACG201.7392025E-594.987454
GATGGCG301.2245437E-894.987444
GATGCCG156.6082476E-494.987444
AGATGCC156.6082476E-494.987443
GATGACG129900.094.8046344
CGAGATG128950.094.801021
AGATGAC129450.094.76733
GAGATGA129550.094.694162
CGAGACG129800.094.6581349
TGACGAG130550.094.114336
ATGACGA130750.094.0430155
ACGAGAC131800.093.113638
GACGAGA133700.091.896987
GGAGATG452.5465852E-1184.529781
GAGATGG353.5695848E-881.417812
CGTGATG301.3524386E-679.2466661
CAAGATG301.3524386E-679.2466661
GTGACGA301.3616645E-679.15625
AGGACGA255.2614057E-575.989955
GGATGAC409.00709E-871.2405853
Produced by FastQC (version 0.11.5)