FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6760
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGATACACGTGACGACGCGTGATCAGCAAAAGCAGGGTGACAAAGA560.8284023668639053No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGTAGTTTTTT500.7396449704142012No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGGTGACAAAGACATAATGGA490.7248520710059172No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTT410.606508875739645No Hit
CGCGCGATACACGTGACGACGCAGAAGCAGGGTGACAAAGACATAATGGA380.5621301775147929No Hit
CGCGCGATACACGTGACGACGCAGCAGAGGCCATGGAGGTTGCTAGTCAG320.4733727810650888No Hit
CGCGCGATACACGTGACGACGCGCGATACGCGTGATCAGTAGAAACAAGG260.38461538461538464No Hit
CGCGCGATACACGTGACGACGCAAGAGCAGGGTGACAAAGACATAATGGA230.3402366863905325No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGTAGATATTGAAAGATGAGT230.3402366863905325No Hit
CGCGCGATACACGTGACGACGCGTGATCAGCAAAAGCAGGTAGATATTGA230.3402366863905325No Hit
CGCGCGATACACGTGACGACGCCTGCCACTTTCTGCTTGGGTATGAGCAT210.3106508875739645No Hit
CGCGCGATACACGTGACGACGCAGGGTGACAAAGACATAATGGATCCAAA210.3106508875739645No Hit
CGCGCGATACACGTGACGACGCAGGGGGACAAAGACATAATGGATCCAAA200.2958579881656805No Hit
CGCGCGATACACGTGACGACGCAGGGGGGCAAAGACATAATGGATCCAAA180.2662721893491124No Hit
CGCGCGATACACGTGACGACGCGCGATACGCGTGATCAGCAAAAGCAGGG170.2514792899408284No Hit
CGCGCGATACACGTGACGACGCCGGACCCAAAGCCTCTACTCAGTGCGAA150.22189349112426035No Hit
CGCGCGATACACGTGACGACGCCACAAAAAGAAATGCTGCTCGCACTAGT140.20710059171597633No Hit
CGCGCGATACACGTGACGACGCAGGGGAAAATAAAAACAACCAAAATGAA140.20710059171597633No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGGTATTTTTC140.20710059171597633No Hit
CGCGCGATACACGTGACGACGCAGGGGGCAAAGACATAATGGATCCAAAC130.19230769230769232No Hit
CGCGCGATACACGTGACGACGCAGGGGTTTAAAATGAATCCAAATCAGAA120.17751479289940827No Hit
CGCGCGATACACGTGACGACGCAGGCCCTCTTTGTATCAGAATGGACCAG110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCGGAGGAAGAAAATTTTGTACGAGGCCAG110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCAGAAGCAGGTAGATATTGAAAGATGAGT110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGGGTTTAAAATGAATCCAAA110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCGTGATCAGCAAAAGCAGGGGTTTAAAAT110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCGGGGGGACAAAGACATAATGGATCCAAA110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCAACAGCAGGGTGACAAAGACATAATGGA110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCAGGGGGCCAAAGACATAATGGATCCAAA110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCGGGCGGGGAAAGATCCTAAGAAAACTGG110.16272189349112426No Hit
CGCGCGATACACGTGACGACGCAGGTACAGAGGCCATGGTCATTTTAAGT100.14792899408284024No Hit
CGCGCGATACACGTGACGACGCAGTAGAAACAAGGGTGTTTTTTATTATT100.14792899408284024No Hit
CGCGCGATACACGTGACGACGCGGGGGACAAAGACATAATGGATCCAAAC90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCAGCAAAAGCAGGGTGACAAAGACATAAT90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCCGGCCAAAAATCACACTGAAGTTCGCTT90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCTTGGGTATGAGCATGGACCAGTCCCTTG90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGAGTTTTTTG90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCAGAAGCAGGGGTTTAAAATGAATCCAAA90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCATAAGCAGGGTGACAAAGACATAATGGA90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCAGGCCCCCTCAAAGCCGAGATCGCACAG90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCCGGGGGACAAAGACATAATGGATCCAAA90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTC90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCATGATCAGCAAAAGCAGGGTGACAAAGA90.1331360946745562No Hit
CGCGCGATACACGTGACGACGCAGTAGAAACAAGGTAGTTTTTTACTCCA80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCATAAGCAGGGGTTTAAAATGAATCCAAA80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCCACAACAAGAAATGCTGCTCGCACTAGT80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCAGAGGCCATGGCCTCTGCTGCTTGCTCA80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCGTGATCGGAAGAGCACACGTCTGAACTC80.1183431952662722Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGCGCGATACACGTGACGACGCAGGGGACAAAGACATAATGGATCCAAAC80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCGAAAGGAGAAATAAATACCTGGAAGAAC80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCAACAGGGGAAAATAAAAACAACCAAAAT80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCCACAGAAAGAAATGCTGCTCGCACTAGT80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCAGCAGCGGCCATGGAGGTTGCTAGTCAG80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCACGCCACAAAAAGAAATGCTGCTCGCAC80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCCCGCGCGATACGCGTGATCAGTAGAAAC80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCAAAAAGAAATGCTGCTCGCACTAGTCCA80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCAAAAGCAGGGTAGATAATCACTCACTGA80.1183431952662722No Hit
CGCGCGATACACGTGACGACGCAAAGCCTCTACTCAGTGCGAAAGCATAC70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCAAGAGCAGGTAGATATTGAAAGATGAGT70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCGGAAGGAGAAATAAATACCTGGAAGAAC70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCAAAGAGGGAATATAATCTCAATATGGAT70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCAGGGGACGAAGACATAATGGATCCAAAC70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCAGGGGGAGAAAGACATAATGGATCCAAA70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCAGCAGCGGCCATGGCCTCTGCTGCTTGC70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCCAGCTCTATGCTGACAAAATGACCATCG70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCGGCGGGGAAAGATCCTAAGAAAACTGGA70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCGTGATCAGTAGAAACAAGGTCGTTTTTA70.10355029585798817No Hit
CGCGCGATACACGTGACGACGCGAGATCGGAAGAGCACACGTCTGAACTC70.10355029585798817Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA6650.095.05
GATACAC6600.095.06
TACACGT6600.095.08
ATACACG6600.095.07
ACACGTG6700.094.2910469
GCGATAC6800.092.205884
GCGCGAT6800.092.205882
CGCGATA6900.091.557973
CGCGCGA6800.091.507351
CACGTGA6650.047.510-11
ACGCAGG550.047.518-19
ACGCAGA451.9281288E-1047.518-19
ACGCAAG252.6802056E-547.518-19
GACGCGT451.9281288E-1047.518-19
GACGCCG509.094947E-1247.518-19
GACGCAG1200.047.518-19
GACGCAA1100.047.518-19
CGCAGGG252.6802056E-547.520-21
GACGACG6700.047.514-15
CGACGCT252.6802056E-547.516-17
Produced by FastQC (version 0.11.5)