FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33512
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTAGTTT5151.5367629505848652No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCCTATCA4111.2264263547386012No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTCTATCA3811.136906182859871No Hit
CGTACTCTCGCATAGACTACTAAGGCCCTCCTTTCAGTCCGTATTTAAAG3441.0264979708761042No Hit
CGTACTCTCGCATAGACTACTACGGCACTCCTTCCGTAGAAGGCCCTCCT3060.9131057531630462No Hit
CGTACTCTCGCATAGACTACTACAGCTCTATGCTGACAAAATGACCATCG1820.543089042730962No Hit
CGTACTCTCGCATAGACTACTAAGGCACTCCTTCCGTAGAAGGCCCTCCT1730.516232991167343No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGTGTTT1730.516232991167343No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTAGATAT1540.4595368823108141No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCAGTTT1360.405824779183576No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGGTGACAA1040.31033659584626405No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCAGATAT1010.301384578658391No Hit
CGTACTCTCGCATAGACTACTACGGCTCTATGCTGACAAAATGACCATCG990.295416567199809No Hit
CGTACTCTCGCATAGACTACTACGGCCCTCCTTTCAGTCCGTATTTAAAG770.22976844115540704No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGAAGTGAGGAATGATGATGTT680.202912389591788No Hit
CGTACTCTCGCATAGACTACTAGCCGCAAATATCATTGGGATCTTGCACT670.199928383862497No Hit
CGTACTCTCGCATAGACTACTACGGCGCTCCTTCCGTAGAAGGCCCTCCT640.190976366674624No Hit
CGTACTCTCGCATAGACTACTAATGGGGGTGCAGATGCAACGGTTCAAGT530.158152303652423No Hit
CGTACTCTCGCATAGACTACTAGGGGCGGGAACAATTAGGTCAGAAGTTT500.14920028646455No Hit
CGTACTCTCGCATAGACTACTAGGGCACTCCTTCCGTAGAAGGCCCTCCT460.137264263547386No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCGTTTT460.137264263547386No Hit
CGTACTCTCGCATAGACTACTAGGGGGCCAAATCAGCATACAACCTACGT450.13428025781809502No Hit
CGTACTCTCGCATAGACTACTACCGCAAATATCATTGGGATCTTGCACTT450.13428025781809502No Hit
CGTACTCTCGCATAGACTACTAGGGCCGCAAATATCATTGGGATCTTGCA440.13129625208880402No Hit
CGTACTCTCGCATAGACTACTACGCCGCAAATATCATTGGGATCTTGCAC440.13129625208880402No Hit
CGTACTCTCGCATAGACTACTAAGGCCCCCTCAAAGCCGAGATCGCACAG430.12831224635951302No Hit
CGTACTCTCGCATAGACTACTAGGGGCCGCAAATATCATTGGGATCTTGC420.125328240630222No Hit
CGTACTCTCGCATAGACTACTAGGGGGAAGAATATCGAAAGGAACAGCAG410.122344234900931No Hit
CGTACTCTCGCATAGACTACTACGGCCGCAAATATCATTGGGATCTTGCA410.122344234900931No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGGGTTTAA400.11936022917164002No Hit
CGTACTCTCGCATAGACTACTAAGCCGCAAATATCATTGGGATCTTGCAC390.116376223442349No Hit
CGTACTCTCGCATAGACTACTACGGGCGGGAACAATTAGGTCAGAAGTTT380.113392217713058No Hit
CGTACTCTCGCATAGACTACTATGCGTGATCAGCAAAAGCAGGTCTATCA380.113392217713058No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTGTTTAA360.10742420625447602No Hit
CGTACTCTCGCATAGACTACTAGAAGGCCCTCCTTTCAGTCCGTATTTAA360.10742420625447602No Hit
CGTACTCTCGCATAGACTACTAATTAGCCTAATATTGCAAATAGGGAATA350.104440200525185No Hit
CGTACTCTCGCATAGACTACTAACGCGTGATCAGTAGAAACAAGGTAGTT340.101456194795894No Hit
CGTACTCTCGCATAGACTACTAAGGCGCTCCTTCCGTAGAAGGCCCTCCT340.101456194795894No Hit
CGTACTCTCGCATAGACTACTAGGAGGGCCTTCTACGGAAGGAGTGCCAA340.101456194795894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCATA156.5614935E-495.08
GGCATAG156.5614935E-495.09
ACTCTGG156.5614935E-495.04
TCGGATA156.5614935E-495.08
CGGATAG156.5614935E-495.09
TGCTCTC156.5614935E-495.03
CGTGCTC156.5614935E-495.01
CTCGGAT156.5614935E-495.07
GCTCTCG156.5614935E-495.04
GTGCTCT156.5614935E-495.02
TCGCATA33100.094.138988
CTCGCAT33000.094.136367
CGCATAG33050.093.993969
TCTCGCA33150.093.85376
ACTCTCG33300.093.716214
TACTCTC34700.090.0720443
GTACTCT34750.089.942452
CGTACTC34800.089.540231
CTCTCGC35750.087.1608355
CTCTGGC200.002056489571.255
Produced by FastQC (version 0.11.5)