FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63205
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATATAGTACTGTCGCTGGGGGGCTATATGAAGCAATTGAGGAGTG3670.5806502650106795No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGAAAGATCCTAAGAAAACTGGA2780.43983862036231314No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAAGATCCTAAGAAAACTGG2270.3591488015188672No Hit
CGAGTATATAGTACTGTCGCTGAAGATAACAGAGAATAGTTTTGAGCAAA2090.33067004192706273No Hit
CGAGTATATAGTACTGTCGCTGGCGGGAACAATTAGGTCAGAAGTTTGAA2080.3290878886164069No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATGGACGAAAAACAAGAAT1960.3101020488885373No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGCCTGACGGGATGATAGAGAGTA1850.29269836247132347No Hit
CGAGTATATAGTACTGTCGCTGAGGGGGCCTGACGGGATGATAGAGAGTA1770.28004113598607705No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAACAATTAGGTCAGAAGTTT1660.26263744956886326No Hit
CGAGTATATAGTACTGTCGCTGGGGGCGGGAACAATTAGGTCAGAAGTTT1490.23574084328771455No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAACAATTAGGTCAGAAGTTTGAA1320.20884423700656593No Hit
CGAGTATATAGTACTGTCGCTGGGGGTGCAGATGCAACGGTTCAAGTGAT1310.20726208369591012No Hit
CGAGTATATAGTACTGTCGCTGAGAAGCAGTAATGAGAATGGGAGACCTC1130.1787833241041057No Hit
CGAGTATATAGTACTGTCGCTGGGGGGCCTGACGGGATGATAGAGAGTAC1130.1787833241041057No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAAGAATATCGAAAGGAACAGCAG1120.17720117079344988No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATATCGAAAGGAACAGCAG1110.1756190174827941No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAGAATATCGAAAGGAACAGCAG1070.16929040424017086No Hit
CGAGTATATAGTACTGTCGCTGGGGGAGGGAAGAATATCGAAAGGAACAG1050.16612609761885927No Hit
CGAGTATATAGTACTGTCGCTGGAGGGAGGAATATCGAAAGGAACAGCAG1040.16454394430820346No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAGGAATATCGAAAGGAACAGCAG990.15663317775492447No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGTGAGAATGGACGAAAAACAAGA950.15030456451230126No Hit
CGAGTATATAGTACTGTCGCTGGAGGGCCTTCTACGGAAGGAGTGCCAAA940.14872241120164545No Hit
CGAGTATATAGTACTGTCGCTGCAAAGACATCTTCAAGTCTCTGTGCGAT890.14081164464836643No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATCTTCGAGCTCTCGGACG880.13922949133771062No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGTCTTCGAGCTCTCGGAC870.1376473380270548No Hit
CGAGTATATAGTACTGTCGCTGCAACTCGTTTGCGGACATGCCAAAGAAA850.13448303140574322No Hit
CGAGTATATAGTACTGTCGCTGGGTTGAATTAATCAGGGGACGACCTAAA840.1329008780950874No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAAAATAAAAACAACCAAAATGA810.12815441816312No Hit
CGAGTATATAGTACTGTCGCTGAGGGAAGAATATCGAAAGGAACAGCAGA790.1249901115418084No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGTGAGGAATGATGATGTT780.1234079582311526No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAACTATTACTGGACCTTGCT780.1234079582311526No Hit
CGAGTATATAGTACTGTCGCTGGGGGCCTGACGGGATGATAGAGAGTACG760.12024365160984099No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAGAATGGACGAAAAACAAGAAT760.12024365160984099No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGCTATATGAAGCAATTGAGGAGT750.11866149829918518No Hit
CGAGTATATAGTACTGTCGCTGCTGGGTTGAATTAATCAGGGGACGACCT740.11707934498852937No Hit
CGAGTATATAGTACTGTCGCTGAGGAGCTGAGGGAGCAATTGAGCTCAGT730.11549719167787359No Hit
CGAGTATATAGTACTGTCGCTGGGGGGCCAAAGTCTATGAGGGAAGAATA720.11391503836721778No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGCCTGACGGGATGATAGAGAGT720.11391503836721778No Hit
CGAGTATATAGTACTGTCGCTGGAGGGGAGAATATCGAAAGGAACAGCAG710.11233288505656197No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGAATATCGAAAGGAACAG660.10442211850328297No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGCCTGACGGGATGATAGAGAG650.10283996519262716No Hit
CGAGTATATAGTACTGTCGCTGGAGGAGGGAAGAATATCGAAAGGAACAG640.10125781188197137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAT201.7051081E-595.288891
TAGGACT254.5736124E-794.987349
TGGTACT254.5736124E-794.987349
GTATCTA156.592264E-494.987344
ATGGTAC254.5736124E-794.987348
AGTATCT156.592264E-494.987343
TAGTAGT156.592264E-494.987349
TATCTAG156.592264E-494.987345
ATATGGT254.5736124E-794.987346
AATATAG156.592264E-494.987345
CGAGTAT62050.094.521051
GAGTATA62150.094.223172
AGTATAT62200.094.147423
ATATAGT62350.094.073276
TAGTACT62150.094.070339
ATAGTAC62150.093.993918
TATAGTA62500.093.695517
GTATATA62400.093.693454
TATATAG62250.093.690335
CGAGGAT452.3646862E-1184.701241
Produced by FastQC (version 0.11.5)