FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92071
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGTCTCTATACGTCTAGCGGGGGTTTTGTTCAGCATCCAGAACTAA2870.3117159583365012No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAAAATGGACGAAGGACAAGG2710.29433806518882166No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAACAGTGACCACAGAAGCTG2420.2628406338586526No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGCCATTCTGTTTTCATGCCTGATT2350.25523780560654274No Hit
CGTCAGTCTCTATACGTCTAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA2030.22048201931118375No Hit
CGTCAGTCTCTATACGTCTAGCCGGCACCATTCTGTTTTCATGCCTGATT2030.22048201931118375No Hit
CGTCAGTCTCTATACGTCTAGCAGGCCCCCTCAAAGCCGAGATCGCGCAG2010.2183097826677238No Hit
CGTCAGTCTCTATACGTCTAGCGAGGCCAAGGGTGTTGCCTCTTCCTTTT1770.19224294294620456No Hit
CGTCAGTCTCTATACGTCTAGCAGAAGCGAATGGGAGTGCAGATGCAGCG1750.19007070630274464No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGAAAATGGACGAAGGACAAGGG1460.1585732749725755No Hit
CGTCAGTCTCTATACGTCTAGCGGGAGTTTTGTTCAGCATCCAGAACTAA1430.15531492000738562No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGAAAATGGACGAAGGACAAGGGT1420.15422880168565561No Hit
CGTCAGTCTCTATACGTCTAGCCCGACCAGTGAGTACCCTTCCCTTTCAA1350.14662597343354586No Hit
CGTCAGTCTCTATACGTCTAGCAGGGGGGGAACAGTGACCACAGAAGCTG1330.1444537367900859No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGCCAAGGAGGTGTCACTAAGCTA1290.14010926350316602No Hit
CGTCAGTCTCTATACGTCTAGCAAAAGCAGGTAGATATTTAAAGATGAGT1240.13467867189451618No Hit
CGTCAGTCTCTATACGTCTAGCCAGAGCAGAAATGAAAAGTGGCGAGAGC1230.1335925535727862No Hit
CGTCAGTCTCTATACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1210.1314203169293263TruSeq Adapter, Index 5 (100% over 37bp)
CGTCAGTCTCTATACGTCTAGCCGCCCAAGATCGAAGGTTCCAGGTTCCA1180.12816196196413637No Hit
CGTCAGTCTCTATACGTCTAGCCGCCTCAAAGCCGAGATCGCGCAGAGAC1170.1270758436424064No Hit
CGTCAGTCTCTATACGTCTAGCATGAGCATGAACCAGTCTCGTGACATTT1130.12273137035548652No Hit
CGTCAGTCTCTATACGTCTAGCCGGACCAGTGAGTACCCTTCCCTTTCAA1100.11947301539029663No Hit
CGTCAGTCTCTATACGTCTAGCCGGAGCAGAAATGAAAAGTGGCGAGAGC1050.11404242378164678No Hit
CGTCAGTCTCTATACGTCTAGCGAGGGGGGAAAATGGACGAAGGACAAGG1040.11295630545991679No Hit
CGTCAGTCTCTATACGTCTAGCAGCAAATATCATTGGGATCTTGCACCTG1000.10861183217299693No Hit
CGTCAGTCTCTATACGTCTAGCGGGGCCAAGGGTGTTGCCTCTTCCTTTT1000.10861183217299693No Hit
CGTCAGTCTCTATACGTCTAGCGGGGACAGAAATTTGAGGAAATAAGGTG980.10643959552953698No Hit
CGTCAGTCTCTATACGTCTAGCCAGCACCATTCTGTTTTCATGCCTGATT970.10535347720780702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC254.5880006E-794.995669
GTCAGTT201.7356762E-594.995662
TCTATAG201.7356762E-594.995669
TGTATAC156.599634E-494.995659
CAGTCTG156.599634E-494.995654
TCTATAA156.599634E-494.995659
CGTCAGT90200.094.564051
CTCTATA90700.094.419618
TCTATAC90750.094.3675849
GTCAGTC90800.094.158682
TCAGTCT91100.094.109313
TCTCTAT91500.093.594087
GTCTCTA91900.093.290086
AGTCTCT92550.092.891495
CAGTCTC94050.091.4099664
TTCTATA301.3577137E-679.163058
TCAGGCT301.3577137E-679.163053
GTCTCGA255.2507778E-575.996526
CTGTATA200.00206837771.246748
AGTCTGT200.00206837771.246745
Produced by FastQC (version 0.11.5)