FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31900
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTAGTCGCGTGTACGTCGCTACGCGTGATCAGCAAAAGCAGGTGGCAC2070.6489028213166145No Hit
CGCTAGTCGCGTGTACGTCGCTACGCGTGATCAGCAAAAGCAGGCGGCAC1930.6050156739811913No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGAAATGAAGCAGGGGGCTGCGC1580.49529780564263326No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGGAAATGAAGCAGGGGGCTGCG1270.39811912225705326No Hit
CGCTAGTCGCGTGTACGTCGCTGCGCGTGATCAGCAAAAGCAGGTGGCAC900.28213166144200624No Hit
CGCTAGTCGCGTGTACGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGT890.2789968652037617No Hit
CGCTAGTCGCGTGTACGTCGCTCCGCGTGATCAGCAAAAGCAGGTGGCAC880.27586206896551724No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGTTGTAGTTGGCGGCAAGATTATTA850.2664576802507837No Hit
CGCTAGTCGCGTGTACGTCGCTGGCCCAGCAGCCATGCCTTCTAAGCAGT730.22884012539184953No Hit
CGCTAGTCGCGTGTACGTCGCTCTTCGAAATGGATGGCACTTTGATTGAA600.18808777429467086No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGAAGAATATCAACAGGAACAGCAG550.1724137931034483No Hit
CGCTAGTCGCGTGTACGTCGCTCCGCGTGATCAGCAAAAGCAGGCGGCAC540.16927899686520376No Hit
CGCTAGTCGCGTGTACGTCGCTGAGGGAAGAATATCAACAGGAACAGCAG530.16614420062695925No Hit
CGCTAGTCGCGTGTACGTCGCTGCGCGTGATCAGCAAAAGCAGGCGGCAC480.15047021943573666No Hit
CGCTAGTCGCGTGTACGTCGCTAGGCGTGATCAGCAAAAGCAGGTGGCAC460.14420062695924765No Hit
CGCTAGTCGCGTGTACGTCGCTGTCAGTAAGTATGCTAGAGTCCCGTTTT430.13479623824451412No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGCAAGAAGGGGATTGAACTCAG430.13479623824451412No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGTGCAGCCCACCCCTCTCATTTG400.12539184952978058No Hit
CGCTAGTCGCGTGTACGTCGCTAAGGGGGCAAGGGGACGTAGTGTTGGTA400.12539184952978058No Hit
CGCTAGTCGCGTGTACGTCGCTATGCGTGATCAGCAAAAGCAGGTGGCAC390.12225705329153606No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGGGAAGCTTCGTGCTGGAGAAAA370.11598746081504703No Hit
CGCTAGTCGCGTGTACGTCGCTCTCAGAATGAAGTGGATGATGGCAATGA350.109717868338558No Hit
CGCTAGTCGCGTGTACGTCGCTGGCGGGGAAATGAAGCAGGGGGCTGCGC350.109717868338558No Hit
CGCTAGTCGCGTGTACGTCGCTCGGCGTGATCAGCAAAAGCAGGTGGCAC350.109717868338558No Hit
CGCTAGTCGCGTGTACGTCGCTAAGACCACTGTGGACCATATGGCCATAA340.10658307210031348No Hit
CGCTAGTCGCGTGTACGTCGCTGGGTGCTGGGGTTGTAGTTGGCGGCAAG340.10658307210031348No Hit
CGCTAGTCGCGTGTACGTCGCTCGGGGGGGAAATGAAGCAGGGGGCTGCG340.10658307210031348No Hit
CGCTAGTCGCGTGTACGTCGCTACGCGTGATCAGTAGAAACAAGGTCGTT330.10344827586206896No Hit
CGCTAGTCGCGTGTACGTCGCTGGGGGGAGAATATCAACAGGAACAGCAG330.10344827586206896No Hit
CGCTAGTCGCGTGTACGTCGCTCGGGGGGAAATGAAGCAGGGGGCTGCGC320.10031347962382445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT32350.092.9362951
GTCGCGT32250.092.78616
TAGTCGC32200.092.782674
AGTCGCG32200.092.782675
CTAGTCG32200.092.782673
GCGTGTA32450.092.653359
GCTAGTC32350.092.646112
CGCGTGT32350.092.499288
TCGCGTG32400.092.356547
CGCTCTT301.470562E-647.49765418-19
CGCTCGT301.470562E-647.49765418-19
CGCTCCG750.047.49765418-19
CGCTCAT301.470562E-647.49765418-19
CTAGGCG301.470562E-647.49765420-21
TCGCTTT301.470562E-647.49765418-19
TCGCTCC1500.047.49765418-19
TTCTAAC301.470562E-647.49765458-59
CTCGGGG750.047.49765420-21
TAGGGGG301.470562E-647.49765422-23
AGCCCGG205.351076E-447.4976558-59
Produced by FastQC (version 0.11.5)