FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55812
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGATATAGTCAGAGAGTAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA1760.31534437038629687No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGGAAAATGGACGAAGGACAAGG1330.23830000716691752No Hit
CGTCGATATAGTCAGAGAGTAGGGGGCCTGACGGGATGATAGAAAGAACG1240.2221744427721637No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGAGCGCCAGGATGCCACAGAAATC1170.2096323371317996No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCAAAAACATAATGGACTCCAACA1090.19529850211424066No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCTATATGAAGCAATCGAGGAGTG900.16125564394753816No Hit
CGTCGATATAGTCAGAGAGTAGCGGCGCGAAGTAGGTACAGATGCAATTG810.14513007955278434No Hit
CGTCGATATAGTCAGAGAGTAGAGGCCCATTAGAGCACATCCAGAAACTG790.1415466207983946No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCAGAAATTTGAGGAAATAAGGTG760.13617143266681No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGCAAAAACATAATGGACTCCAA760.13617143266681No Hit
CGTCGATATAGTCAGAGAGTAGCGGCGAAGTAGGTACAGATGCAATTGTC760.13617143266681No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCGAAAACATAATGGACTCCAACA730.1307962445352254No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGAGCGCCAGGATGCCACAGAAAT710.12721278578083564No Hit
CGTCGATATAGTCAGAGAGTAGAGGCGCGAAGTAGGTACAGATGCAATTG700.1254210564036408No Hit
CGTCGATATAGTCAGAGAGTAGCCGCGAAGTAGGTACAGATGCAATTGTC660.11825413889486132No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGCAAAAACATAATGGACTCCAAC650.11646240951766644No Hit
CGTCGATATAGTCAGAGAGTAGGAGGGGGGAAAATGGACGAAGGACAAGG620.11108722138608185No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGCAATAGTTGGAGAAATTTCAC620.11108722138608185No Hit
CGTCGATATAGTCAGAGAGTAGAGGAGCTAAGAGAGCAATTGAGCTCAGT610.10929549200888698No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCGGAAATTTGAGGAAATAAGGTG590.10571203325449724No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGAAAGCTGGAATCAACAAGGAT570.1021285745001075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT156.5152504E-495.253371
CGTCGAG156.5152504E-495.253371
GATATAT201.729506E-594.9973145
TATAATC156.585429E-494.9973147
TCGGTAT156.585429E-494.9973143
GTCGAGA156.585429E-494.9973142
TAGTCAT156.585429E-494.9973149
ATAGTCG201.729506E-594.9973148
TATTCAG201.729506E-594.9973149
GGTATAG156.585429E-494.9973145
CGGTATA156.585429E-494.9973144
TCGAGAT156.585429E-494.9973143
GTCGGTA156.585429E-494.9973142
CGTCGAT54900.094.385861
GTCGATA55050.094.2207642
TATAGTC54950.094.219357
CGATATA55150.094.136054
ATATAGT54950.094.132916
TAGTCAG54700.093.955299
ATAGTCA55250.093.535828
Produced by FastQC (version 0.11.5)