FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80934
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACTCACGCGCGACACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA4710.5819556675809919No Hit
CGACATACTCACGCGCGACACGATCAGCAAAAGCAGGGTGACAAAAACAT2420.2990090691180468No Hit
CGACATACTCACGCGCGACACGCGGCCAAGGGTGTTGCCTCTTCCTTTTA1990.24587935848963352No Hit
CGACATACTCACGCGCGACACGAACAGAGGTTATAAGAATGATGGAAAGT1840.2273457385029777No Hit
CGACATACTCACGCGCGACACGGGGCCAAGGGTGTTGCCTCTTCCTTTTA1640.2026342451874367No Hit
CGACATACTCACGCGCGACACGATCAGCAAAAGCAGGAGTTTAAAATGAA1150.1420910865643611No Hit
CGACATACTCACGCGCGACACGATGCAATTGTCATTCTAAGTGTCTCGCT1000.1235574665777053No Hit
CGACATACTCACGCGCGACACGGAAACCACTGACTGTCCAGCAGCAAAGT960.11861516791459709No Hit
CGACATACTCACGCGCGACACGAAAAGCAGGGTGACAAAAACATAATGGA920.11367286925148885No Hit
CGACATACTCACGCGCGACACGAGGGGGCCTGACGGGATGATAGAAAGAA920.11367286925148885No Hit
CGACATACTCACGCGCGACACGCGTGATCAGTAGAAACAAGGGTGTTTTT880.10873057058838065No Hit
CGACATACTCACGCGCGACACGACAACATGGATAGAGCAGTTAAACTATA830.1025526972594954No Hit
CGACATACTCACGCGCGACACGGTGGGGAGCGCCAGGATGCCACAGAAAT830.1025526972594954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT156.5961975E-494.9975363
AATCACG156.5961975E-494.9975367
GACTCAC156.5961975E-494.9975366
CTCACGC80900.093.177428
ACTCACG81350.093.1291057
TCACGCG81000.093.062399
TACTCAC81350.093.012336
CGACATA81600.092.665411
GACATAC81950.092.331342
ACATACT82000.092.217113
ATACTCA82150.092.164375
CATACTC81850.092.1539844
TCACGGG353.549576E-881.426459
CTCACGG353.549576E-881.426458
GATACTC452.3828761E-973.886964
AGATACT505.486072E-966.498273
TCACGCT250.00500497956.9985169
CGCGACC301.48455E-647.49876820-21
CGACAAC301.48455E-647.49876820-21
CACGCCT301.48455E-647.49876818-19
Produced by FastQC (version 0.11.5)