FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87250
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA5830.6681948424068768No Hit
CGATAGTCGTACTCAGTGATAGGGGGCCTGACGGGATGATAGAAAGAACG5130.5879656160458453No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAAATGGACGAAGGACAAGG4990.571919770773639No Hit
CGATAGTCGTACTCAGTGATAGCGGCACAGATTGAAAGCGACAGAGAATA3530.4045845272206304No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTATATGAAGCAATCGAGGAGTG3340.3828080229226361No Hit
CGATAGTCGTACTCAGTGATAGCGGCCAAGGGTGTTGCCTCTTCCTTTTA2790.31977077363896844No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGCTCTATTGGGAAAGTG2690.3083094555873926No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAATAGTTGGAGAAATTTCAC2680.307163323782235No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGAGTTGAACCAAGATGC2580.295702005730659No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGAAAATGGACGAAGGACAAGGG1980.22693409742120343No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCAGATTGAAAGCGACAGAGAATA1750.20057306590257878No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATTAGAGCACATCCAGAAACTG1320.1512893982808023No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATGCAACTGGCAAGTGCACCAG1120.12836676217765042No Hit
CGATAGTCGTACTCAGTGATAGGGTGGTTAATTGAAGAAATGCGGCACAG1100.12607449856733524No Hit
CGATAGTCGTACTCAGTGATAGGGGGGAAGAATATCAACAGGAACAGCAG1090.12492836676217765No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGCAAAAACATAATGGACTCCAAC1080.12378223495702007No Hit
CGATAGTCGTACTCAGTGATAGGAGGGGGGAAAATGGACGAAGGACAAGG1050.12034383954154727No Hit
CGATAGTCGTACTCAGTGATAGAGGGGGCCTGACGGGATGATAGAAAGAA1010.11575931232091692No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAAAAACATAATGGACTCCAACA1000.1146131805157593No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTGGTGTTTATAGCACCCTTGGG940.10773638968481375No Hit
CGATAGTCGTACTCAGTGATAGGGGGCGGGAAGTCTTCGAGCTCTCGGAC930.10659025787965615No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCCTGACGGGATGATAGAAAGAAC920.10544412607449856No Hit
CGATAGTCGTACTCAGTGATAGGGGGCCAAGGAGGTGTCACTAAGCTATT910.10429799426934097No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAGAAATTTGAGGAAATAAGGTG910.10429799426934097No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAAAATTAAACAGAGAAGAAATA900.10315186246418338No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAAAAACATAATGGACTCCAA900.10315186246418338No Hit
CGATAGTCGTACTCAGTGATAGCGAGGCCAAGGGTGTTGCCTCTTCCTTT880.10085959885386821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT201.7349279E-594.998287
TTGTACT201.7349279E-594.998287
AGGCGTA353.255991E-1094.998285
AGACGTA353.255991E-1094.998285
GTTGTAC201.7349279E-594.998286
CGTAGTC201.7349279E-594.998288
GTCGTAG201.7349279E-594.998286
GTACTCC450.094.998289
CGTACTG201.7349279E-594.998288
TCGTAGT201.7349279E-594.998287
TGTACTC201.7349279E-594.998288
GTAGTCG353.255991E-1094.998283
GTAGTCA201.7349279E-594.998289
GGTAGTC353.255991E-1094.998282
GGTACTC254.5852175E-794.9982768
CGAACTC156.597744E-494.9982768
GTCGAAC156.597744E-494.9982766
TCGGACT301.2187229E-894.9982767
CGTACAC156.597744E-494.9982768
TCGTACA156.597744E-494.9982767
Produced by FastQC (version 0.11.5)